Fomin Victor P, Kronbergs Andris, Gunst Susan, Tang Dale, Simirskii Vladimir, Hoffman Matthew, Duncan Randall L
Department of Biological Sciences, University of Delaware, Newark, Delaware 19716, USA.
Reprod Sci. 2009 Jan;16(1):71-9. doi: 10.1177/1933719108324892. Epub 2008 Dec 15.
Recent findings implicate protein kinase C in regulation of contraction of uterine muscle (myometrium). However, the role of protein kinase C isoforms in myometrial contraction remains uncertain. Therefore, this study examined protein kinase Calpha's role in regulation of contraction and intracellular calcium concentration (Ca2+) of myometrium from term pregnant women. The authors demonstrated that protein kinase Calpha inhibitor Go6976 decreased the amplitude of potassium chloride-induced myometrial contractions in a time-dependent manner. The treatment of the myometrial strips with protein kinase Calpha-specific antisense oligodeoxynucleotides decreased the potassium chloride-induced contraction and Ca2+ response to 39.3% + 6.8% and 50.0% + 3.3%, respectively, compared to control. The sense oligonucleotides treatment did not significantly change the potassium chloride responses (89.8% + 6.8% and 93.9% + 4.5% of the control for the contraction and Ca2+, respectively). These data, coupled with the observation that protein kinase Calpha levels are elevated in the pregnant myometrium, suggest the involvement of protein kinase Calpha in regulation of human uterine contraction.
最近的研究结果表明蛋白激酶C参与子宫肌层（子宫平滑肌）收缩的调节。然而，蛋白激酶C亚型在子宫肌层收缩中的作用仍不明确。因此，本研究探讨了蛋白激酶Cα在足月妊娠妇女子宫肌层收缩调节及细胞内钙浓度（[Ca2+]i）中的作用。作者证明，蛋白激酶Cα抑制剂Go6976以时间依赖性方式降低氯化钾诱导的子宫肌层收缩幅度。用蛋白激酶Cα特异性反义寡脱氧核苷酸处理子宫肌层条，与对照组相比，氯化钾诱导的收缩和[Ca2+]i反应分别降低至39.3% + 6.8%和50.0% + 3.3%。正义寡核苷酸处理对氯化钾反应无显著影响（收缩和[Ca2+]i分别为对照组的89.8% + 6.8%和93.9% + 4.5%）。这些数据，再加上妊娠子宫肌层中蛋白激酶Cα水平升高的观察结果，表明蛋白激酶Cα参与人类子宫收缩的调节。
