Kyratsous Christos A, Silverstein Saul J
Department of Microbiology, College of Physicians and Surgeons, Columbia University, 701 West 168th Street, New York, NY 10032, USA.
Proc Natl Acad Sci U S A. 2008 Dec 30;105(52):20912-7. doi: 10.1073/pnas.0810656105. Epub 2008 Dec 16.
Viruses as obligate intracellular parasites use host cell proteins to ensure efficient replication and spread. Cellular proteins are required for several stages of a virus life cycle. Here, we identify BAG3, a co-chaperone, as a regulator of herpes virus immediate early gene expression. We report that a herpes simplex virus lacking the gene encoding a potent transcriptional activator, ICP0, is compromised for replication in cells silenced for BAG3 in a multiplicity of infection-dependent manner. We also show a requirement for BAG3 to augment virus gene expression and demonstrate that the co-chaperone acts independently of promyelocytic leukemia to increase herpes simplex virus replication.
病毒作为专性细胞内寄生物,利用宿主细胞蛋白来确保高效复制和传播。细胞蛋白在病毒生命周期的多个阶段都是必需的。在此,我们鉴定出一种共伴侣蛋白BAG3作为疱疹病毒立即早期基因表达的调节因子。我们报告称,一种缺乏编码强效转录激活因子ICP0基因的单纯疱疹病毒,在以感染复数依赖性方式沉默BAG3的细胞中复制受损。我们还表明,BAG3是增强病毒基因表达所必需的,并证明这种共伴侣蛋白独立于早幼粒细胞白血病发挥作用以增加单纯疱疹病毒的复制。