Pontual Maria Luiza dos Anjos, Tuji Fabrício Mesquita, Barros Silvana Pereira, Bóscolo Frab Norberto, Novaes Pedro Duarte, de Almeida Solange Maria
Department of Clinic and Social Dentistry, School of Dentistry, Federal University of Paraíba, João Pessoa, PB, Brazil.
J Appl Oral Sci. 2007 Jun;15(3):162-8. doi: 10.1590/s1678-77572007000300003.
The aim of this study was to evaluate the radioprotector effect of sodium selenite on the ultrastructure of submandibular glands in rats. Fifty-seven male albino Wistar rats were randomized to 4 groups: control, irradiated, sodium selenite and irradiated/sodium selenite. The animals in the sodium selenite and irradiated/sodium selenite groups received intraperitoneal injections of sodium selenite (0.5 mg/kg body weight) 24 h before irradiation. The animals belonging to the irradiated and irradiated/sodium selenite groups were submitted to 15 Gy of gamma radiation in the head and neck region. The submandibular glands were removed at 4, 8, 12, 24, 48 and 72 h after irradiation. The ionizing radiation induced damage to the secretory cells, especially the serous cells, right from the first period. Vacuolization, lysis of cytoplasmic inclusions and nuclear alterations occurred. The sodium selenite group also presented cellular alterations in the study periods, but with less damage compared to that caused by radiation. There was greater similarity between the irradiated/sodium selenite group and the control group than with the other groups treated in all study periods. Despite the alterations observed in the sodium selenite group, sodium selenite presented a radioprotective action on the secretory cells of submandibular glands.
本研究的目的是评估亚硒酸钠对大鼠下颌下腺超微结构的辐射防护作用。57只雄性白化Wistar大鼠被随机分为4组：对照组、辐照组、亚硒酸钠组和辐照/亚硒酸钠组。亚硒酸钠组和辐照/亚硒酸钠组的动物在辐照前24小时腹腔注射亚硒酸钠（0.5mg/kg体重）。辐照组和辐照/亚硒酸钠组的动物接受头颈部15Gy的γ射线照射。在辐照后4、8、12、24、48和72小时取下颌下腺。电离辐射从最初阶段就对分泌细胞，尤其是浆液性细胞造成损伤。出现了空泡化、细胞质内含物溶解和核改变。亚硒酸钠组在研究期间也出现了细胞改变，但与辐射造成的损伤相比损伤较小。在所有研究阶段，辐照/亚硒酸钠组与对照组的相似性大于与其他处理组的相似性。尽管在亚硒酸钠组中观察到了改变，但亚硒酸钠对下颌下腺的分泌细胞具有辐射防护作用。
