Sperandio Cristina Berthold, Silveira Luiz Fernando Machado, de Araújo Lenita Aver, Martos Josué, Malshe Ashwin
Department of Clinics, Dental School, Federal University of Pelotas, Pelotas, RS, Brazil.
J Appl Oral Sci. 2008 Jan-Feb;16(1):59-63. doi: 10.1590/s1678-77572008000100012.
The purpose of this study was to analyze the inflammatory response of dog's periapical tissues to 17% trisodium EDTA salt (pH 8.0) and 1% citric acid (pH 2.0). Saline was used as a control. Six adult dogs were used as the biological model of the study. The experimental units comprised 56 roots of mandibular molars (first and second) and premolars (first, second and third). After coronal opening, pulpectomy and root canal instrumentation were performed using the above-mentioned irrigating solutions. After 24 and 48 hours, the animals were euthanized and the teeth and their supporting tissues were removed and histologically processed. The sections were stained with hematoxylin and eosin and analyzed histopathologically with a light microscope at x100 magnification. The histological analysis focused on the occurrence of acute inflammatory response. The presence of swelling, vasodilatation and inflammatory cells were evaluated and the degree of inflammation was determined for each case. Data were analyzed by Fisher's exact test using the SPSS software with a confidence interval of 95% (p<0.05). 17% EDTA and 1% citric acid caused inflammatory responses in dog's periapical tissues with no significant differences to each other or to saline (control) at either the 24-hour (p=0.482) or 48-hour (p=0.377) periods. It may be concluded that the inflammatory response was of mild intensity for the tested substances.
本研究的目的是分析犬根尖周组织对17%乙二胺四乙酸三钠（pH 8.0）和1%柠檬酸（pH 2.0）的炎症反应。使用生理盐水作为对照。六只成年犬作为该研究的生物学模型。实验单位包括56颗下颌磨牙（第一和第二磨牙）和前磨牙（第一、第二和第三前磨牙）的牙根。在开髓后，使用上述冲洗液进行牙髓摘除术和根管预备。在24小时和48小时后，对动物实施安乐死，并取出牙齿及其支持组织进行组织学处理。切片用苏木精和伊红染色，并在100倍放大率下用光学显微镜进行组织病理学分析。组织学分析聚焦于急性炎症反应的发生情况。评估肿胀、血管扩张和炎症细胞的存在情况，并确定每个病例的炎症程度。使用SPSS软件通过Fisher精确检验分析数据，置信区间为95%（p<0.05）。17%乙二胺四乙酸和1%柠檬酸在犬根尖周组织中引起炎症反应，在24小时（p=0.482）或48小时（p=0.377）时，彼此之间或与生理盐水（对照）相比均无显著差异。可以得出结论，对于所测试的物质，炎症反应强度较轻。
