Tremblay E, Zini S, Ben-Ari Y
Laboratoire de Neurobiologie et Physiopathologie du développement, INSERM U29, Paris, France.
Neurosci Lett. 1991 Jun 10;127(1):21-4. doi: 10.1016/0304-3940(91)90884-v.
[3H]Glibenclamide, a potent ATP-sensitive K+ channel blocker, is a specific ligand for the identification of the sulfonylureas receptors which are closely related to ATP-sensitive K+ channels. In order to determine the pre- or postsynaptic localization of these receptors in the rat hippocampus, we studied the effects of selective kainate or colchicine-induced lesions on the regional distribution of [3H]glibenclamide binding. A decreased binding was found in the following conditions: in CA3, after destruction of the mossy fiber terminals but not of the CA3 cells; in CA1, after destruction of the CA1 cells but not of the Schaffer collaterals; in the fascia dentata, after destruction of the granule cells. These results suggest that glibenclamide binding sites are mainly localized on the mossy fibers in CA3, and on the granular and pyramidal neurons in the fascia dentata and CA1, respectively.
[3H]格列本脲是一种强效的ATP敏感性钾通道阻滞剂，是用于识别与ATP敏感性钾通道密切相关的磺酰脲类受体的特异性配体。为了确定这些受体在大鼠海马体中的突触前或突触后定位，我们研究了选择性谷氨酸钾或秋水仙碱诱导的损伤对[3H]格列本脲结合区域分布的影响。在以下情况下发现结合减少：在CA3区，苔藓纤维终末被破坏但CA3细胞未被破坏后；在CA1区，CA1细胞被破坏但谢弗侧支未被破坏后；在齿状回，颗粒细胞被破坏后。这些结果表明，格列本脲结合位点主要分别位于CA3区的苔藓纤维上，以及齿状回和CA1区的颗粒神经元和锥体细胞上。
