Yoo Ki-Yeon, Park Ok Kyu, Yu Jiatian, Yan Bingchun, Li Hua, Lee Choong Hyun, Choi Jung Hoon, Kim Dae Won, Hwang In Koo, Won Moo-Ho
Department of Anatomy and Neurobiology, College of Medicine, Hallym University, Chuncheon, 200-702, South Korea.
Cell Mol Neurobiol. 2009 May;29(3):413-21. doi: 10.1007/s10571-008-9333-7. Epub 2008 Dec 17.
Oxidative stress is one of predisposing factors to age-related neurodegeneration in the brain. In particular, thiol-containing groups are susceptible to oxidative stress, which induces the formation of the disulfide bond and/or hyperoxidized form of thiol-containing proteins. We observed the protein thiol levels in the hippocampal homogenates and also investigated changes in hyperoxidized form of peroxiredoxin (Prx-SO(3)) immunoreactivity and proteins levels in the gerbil hippocampal subregions during normal aging. Levels of total thiol, non-protein thiol, and protein thiol were decreased in the hippocampal homogenates with age. At post-natal month 1 (PM 1), pyramidal and non-pyramidal cells in the hippocampal CA1 region (CA1) showed Prx-SO(3) immunoreactivity. Prx-SO(3) immunoreactivity in the cells was decreased by PM 12, thereafter, Prx-SO(3) immunoreactivity in the cells increased again with age. In the CA2/3, Prx-SO(3) immunoreactivity in pyramidal cells was not significantly changed; however, the immunoreactivity in pyramidal cells was very low at PM 12. Prx-SO(3) immunoreactivity in the dentate gyrus (DG) was distinctly changed during aging. At PM 1, Prx-SO(3) immunoreactivity in granule and polymorphic cells was weak and strong, respectively. The immunoreactivity in the neurons was decreased with age, not shown in any neurons at PM 12. Thereafter, Prx-SO(3) immunoreactivity increased again with age. In addition, Prx-SO(3) protein level in the hippocampus was lowest at PM 12. These results suggest that thiol-containing proteins are changed during aging and Prx-SO(3) immunoreactivity was different according to cells in the hippocampal subregion during aging.
氧化应激是大脑中与年龄相关的神经退行性变的诱发因素之一。特别是，含硫醇基团易受氧化应激影响，氧化应激会诱导含硫醇蛋白质形成二硫键和/或超氧化形式。我们观察了海马匀浆中的蛋白质硫醇水平，并研究了正常衰老过程中沙鼠海马亚区过氧化物还原酶超氧化形式（Prx-SO(3)）的免疫反应性变化和蛋白质水平。随着年龄增长，海马匀浆中的总硫醇、非蛋白质硫醇和蛋白质硫醇水平均下降。出生后第1个月（PM 1），海马CA1区（CA1）的锥体细胞和非锥体细胞显示出Prx-SO(3)免疫反应性。到PM 12时，细胞中的Prx-SO(3)免疫反应性降低，此后，细胞中的Prx-SO(3)免疫反应性又随年龄增长而增加。在CA2/3区，锥体细胞中的Prx-SO(3)免疫反应性没有显著变化；然而，在PM 12时，锥体细胞中的免疫反应性非常低。齿状回（DG）中的Prx-SO(3)免疫反应性在衰老过程中明显变化。在PM 1时，颗粒细胞和多形细胞中的Prx-SO(3)免疫反应性分别较弱和较强。神经元中的免疫反应性随年龄增长而降低，在PM 12时任何神经元中均未显示。此后，Prx-SO(3)免疫反应性又随年龄增长而增加。此外，海马中的Prx-SO(3)蛋白水平在PM 12时最低。这些结果表明，含硫醇蛋白质在衰老过程中发生变化，并且在衰老过程中，海马亚区不同细胞的Prx-SO(3)免疫反应性有所不同。
