成年沙鼠海马齿状回颗粒细胞层中胰高血糖素样肽-1 受体免疫反应性降低。
Decreased glucagon-like peptide-1 receptor immunoreactivity in the dentate granule cell layer from adult in the gerbil hippocampus.
机构信息
Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Research Institute for Veterinary Science, Seoul National University, South Korea.
出版信息
Cell Mol Neurobiol. 2011 Apr;31(3):345-50. doi: 10.1007/s10571-010-9632-7. Epub 2010 Dec 9.
In this study, we investigated age-related changes in glucagon-like peptide-1 receptor (GLP-1R) immunoreactivity and its protein levels in the gerbil hippocampus during normal aging. In the postnatal month 3 (PM 3) group, GLP-1R immunoreaction was well observed in neurons, especially pyramidal and non-pyramidal cells in the hippocampus proper, and granule and polymorphic cells in the dentate gyrus. In the hippocampus proper, GLP-1R immunoreactivity in neurons was maintained until PM 24. In the dentate gyrus, however, GLP-1R immunoreactivity in granule cells, not polymorphic cells, was hardly detected from PM 6. Western blot analysis also showed that age-dependent change patterns in GLP-1R protein levels in the gerbil hippocampus were similar to the immunohistochemical changes. These results indicate that GLP-1R immunoreactivity was markedly decreased in dentate granule cells from PM 6, showing that GLP-1R immunoreactivity and its protein levels were decreased in the adult and aged gerbil hippocampus.
在这项研究中，我们研究了正常衰老过程中沙鼠海马中海马 GLP-1 受体（GLP-1R）免疫反应及其蛋白水平的年龄相关性变化。在出生后第 3 个月（PM 3）组中，GLP-1R 免疫反应在神经元中得到了很好的观察，特别是在海马的锥体和非锥体细胞中，以及在齿状回的颗粒和多形细胞中。在海马中，神经元中的 GLP-1R 免疫反应一直持续到 PM 24。然而，在齿状回中，从 PM 6 开始，颗粒细胞而非多形细胞中的 GLP-1R 免疫反应几乎无法检测到。Western blot 分析还表明，沙鼠海马中海马 GLP-1R 蛋白水平的年龄依赖性变化模式与免疫组织化学变化相似。这些结果表明，从 PM 6 开始，齿状回颗粒细胞中的 GLP-1R 免疫反应明显减少，表明 GLP-1R 免疫反应及其蛋白水平在成年和老年沙鼠海马中降低。
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