Emmerechts Gert, Maes Liesbeth, Herdewijn Piet, Anné Jozef, Rozenski Jef
Laboratory for Medicinal Chemistry, Rega Institute for Medical Research, Katholieke Universiteit Leuven, Minderbroedersstraat 10, Leuven, Belgium.
Chem Biodivers. 2008 Dec;5(12):2640-53. doi: 10.1002/cbdv.200890218.
It is generally accepted that posttranscriptional modifications in RNA play a role in the fine-tuning of RNA function and the maintenance of RNA structure. This article describes the characterization of the posttranscriptional modifications in Legionella pneumophila 16S rRNA by mass spectrometry and reverse transcriptase assays. Eight modified nucleotides were identified and mapped in the 16S rRNA sequence. Situation of these data in relation to general 16S rRNA modification patterns shows that L. pneumophila is relatively less modified, and that the majority of the L. pneumophila 16S rRNA modifications are conserved among the bacteria characterized so far (Escherichia coli, Clostridium acetobutylicum, Thermus thermophilus, and Thermotoga maritima).
人们普遍认为，RNA的转录后修饰在RNA功能的微调及RNA结构的维持中发挥作用。本文描述了通过质谱分析和逆转录酶测定法对嗜肺军团菌16S rRNA转录后修饰的表征。在16S rRNA序列中鉴定并定位了8个修饰核苷酸。这些数据与一般16S rRNA修饰模式的关系表明，嗜肺军团菌的修饰相对较少，并且嗜肺军团菌16S rRNA的大多数修饰在目前已表征的细菌（大肠杆菌、丙酮丁醇梭菌、嗜热栖热菌和海栖热袍菌）中是保守的。
