Bencini M A, van den Brule A J C, Claas E C J, Hermans M H A, Melchers W J G, Noordhoek G T, Salimans M M M, Schirm J, Vink C, van der Zee A, Jansen R
Regional Public Health Laboratory Kennemerland, Boerhaavelaan 26, 2035 RC Haarlem, The Netherlands.
J Clin Microbiol. 2007 Oct;45(10):3390-2. doi: 10.1128/JCM.00505-07. Epub 2007 Aug 1.
Legionellosis can be diagnosed by PCR using sputum samples. In this report, the methods of nine laboratories for 12 sputum samples with Legionella pneumophila and Legionella longbeachae are compared. We conclude that (i) liquefaction prevents PCR inhibition, (ii) the employed mip gene PCRs detected L. pneumophila only, and (iii) the 16S rRNA gene PCR detected both Legionella species and is preferred for the diagnosis of legionellosis.
可通过使用痰液样本的聚合酶链反应（PCR）诊断军团菌病。在本报告中，比较了九个实验室对12份含有嗜肺军团菌和长滩军团菌的痰液样本的检测方法。我们得出以下结论：（i）液化可防止PCR抑制；（ii）所采用的巨噬细胞感染增强蛋白（mip）基因PCR仅检测到嗜肺军团菌；（iii）16S核糖体RNA（rRNA）基因PCR可检测到两种军团菌属，是诊断军团菌病的首选方法。
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