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本文引用的文献

1
Comparison of five methods for extraction of Legionella pneumophila from respiratory specimens.从呼吸道标本中提取嗜肺军团菌的五种方法的比较。
J Clin Microbiol. 2004 Dec;42(12):5913-6. doi: 10.1128/JCM.42.12.5913-5916.2004.
2
Laboratory diagnosis of legionnaires' disease due to Legionella pneumophila serogroup 1: comparison of phenotypic and genotypic methods.嗜肺军团菌血清1型所致军团病的实验室诊断：表型与基因型方法的比较
J Med Microbiol. 2004 Mar;53(Pt 3):183-187. doi: 10.1099/jmm.0.05464-0.
3
Real-time PCR assay targets the 23S-5S spacer for direct detection and differentiation of Legionella spp. and Legionella pneumophila.实时聚合酶链反应（PCR）检测法以23S - 5S间隔区为靶点，用于直接检测和区分嗜肺军团菌属及嗜肺军团菌。
J Clin Microbiol. 2003 Oct;41(10):4815-6. doi: 10.1128/JCM.41.10.4815-4816.2003.
4
Development and clinical evaluation of an internally controlled, single-tube multiplex real-time PCR assay for detection of Legionella pneumophila and other Legionella species.一种用于检测嗜肺军团菌及其他军团菌属的内控单管多重实时荧光定量PCR检测方法的研发与临床评估
J Clin Microbiol. 2003 Sep;41(9):4016-21. doi: 10.1128/JCM.41.9.4016-4021.2003.
5
Detection of Legionella pneumophila by real-time PCR for the mip gene.通过实时聚合酶链反应检测嗜肺军团菌的mip基因。
J Clin Microbiol. 2003 Jul;41(7):3327-30. doi: 10.1128/JCM.41.7.3327-3330.2003.
6
Direct detection and differentiation of Legionella spp. and Legionella pneumophila in clinical specimens by dual-color real-time PCR and melting curve analysis.通过双色实时荧光定量PCR和熔解曲线分析直接检测和鉴别临床标本中的嗜肺军团菌及其他军团菌属细菌。
J Clin Microbiol. 2002 Oct;40(10):3814-7. doi: 10.1128/JCM.40.10.3814-3817.2002.
7
Novel PCR-probe assay for detection of and discrimination between Legionella pneumophila and other Legionella species in clinical samples.用于检测和区分临床样本中嗜肺军团菌与其他军团菌属的新型聚合酶链反应-探针检测法。
J Clin Microbiol. 2002 Mar;40(3):1124-5. doi: 10.1128/JCM.40.3.1124-1127.2002.
8
Detection of legionellae in hospital water samples by quantitative real-time LightCycler PCR.采用定量实时荧光定量PCR技术检测医院水样中的军团菌。
Appl Environ Microbiol. 2001 Sep;67(9):3985-93. doi: 10.1128/AEM.67.9.3985-3993.2001.
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Detection of Legionella pneumophila using a real-time PCR hybridization assay.使用实时PCR杂交分析法检测嗜肺军团菌。
J Clin Microbiol. 2000 Nov;38(11):4215-8. doi: 10.1128/JCM.38.11.4215-4218.2000.
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A PCR test for the identification and discrimination of Legionella longbeachae serogroups 1 and 2.用于鉴定和区分长滩军团菌血清群1和血清群2的聚合酶链反应（PCR）检测
J Microbiol Methods. 1999 Sep;37(3):245-53. doi: 10.1016/s0167-7012(99)00070-6.

痰标本中军团菌属检测分子方法的多中心比较

Multicenter comparison of molecular methods for detection of Legionella spp. in sputum samples.

作者信息

Bencini M A, van den Brule A J C, Claas E C J, Hermans M H A, Melchers W J G, Noordhoek G T, Salimans M M M, Schirm J, Vink C, van der Zee A, Jansen R

机构信息

Regional Public Health Laboratory Kennemerland, Boerhaavelaan 26, 2035 RC Haarlem, The Netherlands.

出版信息

J Clin Microbiol. 2007 Oct;45(10):3390-2. doi: 10.1128/JCM.00505-07. Epub 2007 Aug 1.

DOI:10.1128/JCM.00505-07
PMID:17670926
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2045317/
Abstract

Legionellosis can be diagnosed by PCR using sputum samples. In this report, the methods of nine laboratories for 12 sputum samples with Legionella pneumophila and Legionella longbeachae are compared. We conclude that (i) liquefaction prevents PCR inhibition, (ii) the employed mip gene PCRs detected L. pneumophila only, and (iii) the 16S rRNA gene PCR detected both Legionella species and is preferred for the diagnosis of legionellosis.

摘要

可通过使用痰液样本的聚合酶链反应（PCR）诊断军团菌病。在本报告中，比较了九个实验室对12份含有嗜肺军团菌和长滩军团菌的痰液样本的检测方法。我们得出以下结论：（i）液化可防止PCR抑制；（ii）所采用的巨噬细胞感染增强蛋白（mip）基因PCR仅检测到嗜肺军团菌；（iii）16S核糖体RNA（rRNA）基因PCR可检测到两种军团菌属，是诊断军团菌病的首选方法。