Ogushi Yuko, Sakai Shinji, Kawakami Koei
Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
Macromol Biosci. 2009 Mar 10;9(3):262-7. doi: 10.1002/mabi.200800263.
The effect of Ph-OH group content on gelation time, mechanical properties, hydrophobicity, and cellular adhesiveness of hydrogels produced from carboxymethylcellulose derivatives is investigated. A higher Ph-OH group content induces faster gelation and yields more brittle and hydrophobic gels. After 4 h of seeding, a larger number of L929 fibroblasts adhere to the hydrogel of the CMC-Ph that contains 15.4 Ph-OH groups per 100 repeat units of uronic acid (97% adhesion rate) than to the gel of CMC-Ph with only 8.4 Ph-OH groups (62% adhesion rate). The results demonstrate that controlling the Ph-OH group content is an effective and useful way to control cellular adhesion and proliferation on the hydrogels, as well as gelation time and mechanical properties of the gels.
研究了Ph-OH基团含量对由羧甲基纤维素衍生物制备的水凝胶的凝胶化时间、力学性能、疏水性和细胞粘附性的影响。较高的Ph-OH基团含量会导致更快的凝胶化，并产生更脆且更疏水的凝胶。接种4小时后，与每100个糖醛酸重复单元仅含8.4个Ph-OH基团的CMC-Ph凝胶（粘附率62%）相比，更多的L929成纤维细胞粘附于每100个糖醛酸重复单元含15.4个Ph-OH基团的CMC-Ph水凝胶（粘附率97%）。结果表明，控制Ph-OH基团含量是控制水凝胶上细胞粘附和增殖以及凝胶的凝胶化时间和力学性能的有效且有用的方法。
