Department of Materials Science and Engineering, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama, Toyonaka, Osaka 560-8531, Japan.
Biomolecules. 2019 Aug 5;9(8):342. doi: 10.3390/biom9080342.
Composite hydrogels of hyaluronic acid and gelatin attract great attention in biomedical fields. In particular, the composite hydrogels obtained through processes that are mild for cells are useful in tissue engineering. In this study, hyaluronic acid/gelatin composite hydrogels obtained through a blue light-induced gelation that is mild for mammalian cells were studied for the effect of the content of each polymer in the precursor solution on gelation, properties of resultant hydrogels, and behaviors of human adipose stem cells laden in the hydrogels. Control of the content enabled gelation in less than 20 s, and also enabled hydrogels to be obtained with 0.5-1.2 kPa Young's modulus. Human adipose stem cells were more elongated in hydrogels with a higher rather than lower content of hyaluronic acid. Stem cell marker genes, , , and , were expressed more in the cells in the composite hydrogels with a higher content of hyaluronic acid compared with those in the hydrogel composed of gelatin alone and on tissue culture dishes. These results are useful for designing conditions for using gelatin/hyaluronic acid composite hydrogels obtained through blue light-induced gelation suitable for tissue engineering applications.
透明质酸/明胶复合水凝胶在生物医学领域受到广泛关注。特别是通过对细胞温和的过程获得的复合水凝胶在组织工程中非常有用。在这项研究中，研究了通过对哺乳动物细胞温和的蓝光诱导凝胶化获得的透明质酸/明胶复合水凝胶，探讨了前体溶液中每种聚合物含量对凝胶化、水凝胶性能以及负载在水凝胶中的人脂肪干细胞行为的影响。控制含量可以在不到 20 秒内实现凝胶化，并获得杨氏模量为 0.5-1.2 kPa 的水凝胶。水凝胶中透明质酸含量越高，人脂肪干细胞的形态越细长。与单独的明胶水凝胶和组织培养皿相比，在透明质酸含量较高的复合水凝胶中，干细胞标记基因、、、和的表达更多。这些结果有助于设计通过蓝光诱导凝胶化获得的明胶/透明质酸复合水凝胶用于组织工程应用的条件。
