Prabaharan Mani, Grailer Jamison J, Pilla Srikanth, Steeber Douglas A, Gong Shaoqin
Department of Mechanical Engineering, University of Wisconsin-Milwaukee, Milwaukee, WI 53211, USA.
Macromol Biosci. 2009 May 13;9(5):515-24. doi: 10.1002/mabi.200800269.
A novel type of biodegradable/biocompatible amphiphilic hyperbranched copolymer (H40-PLA-b-MPEG) was synthesized. Its micellar properties were studied by DLS, fluorescence spectroscopy and TEM. The drug release profile showed that the H40-PLA-b-MPEG micelles provide an initial burst release, followed by a sustained release of the entrapped hydrophobic model drug over a period of 4 to 58 h. The copolymer degraded hydrolytically within 6 weeks under physiological conditions. The MTT assay showed no obvious cytotoxicity against a human endothelial cell line at a concentration range of 0-400 microg x mL(-1). These results indicate that the H40-PLA-b-MPEG micelles have great potential as hydrophobic drug delivery carriers.
合成了一种新型的可生物降解/生物相容的两亲性超支化共聚物（H40-PLA-b-MPEG）。通过动态光散射（DLS）、荧光光谱和透射电子显微镜（TEM）研究了其胶束性质。药物释放曲线表明，H40-PLA-b-MPEG胶束呈现初始突释，随后在4至58小时内持续释放包封的疏水模型药物。该共聚物在生理条件下6周内可水解降解。MTT法检测显示，在0-400μg x mL(-1)浓度范围内，对人内皮细胞系无明显细胞毒性。这些结果表明，H40-PLA-b-MPEG胶束作为疏水药物递送载体具有巨大潜力。
