Afinjuomo Franklin, Fouladian Paris, Parikh Ankit, Barclay Thomas G, Song Yunmei, Garg Sanjay
School of Pharmacy and Medical Sciences, University of South Australia, Adelaide, South Australia 5001, Australia.
Pharmaceutics. 2019 Jul 22;11(7):356. doi: 10.3390/pharmaceutics11070356.
Inulin-based hydrogels are useful carriers for the delivery of drugs in the colon-targeted system and in other biomedical applications. In this project, inulin hydrogels were fabricated by crosslinking oxidized inulin with adipic acid dihydrazide (AAD) without the use of a catalyst or initiator. The physicochemical properties of the obtained hydrogels were further characterized using different techniques, such as swelling experiments, in vitro drug release, degradation, and biocompatibility tests. The crosslinking was confirmed with Fourier transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA), and differential scanning calorimetry (DSC). In vitro releases of 5-fluorouracil (5FU) from the various inulin hydrogels was enhanced in acidic conditions (pH 5) compared with physiological pH (pH 7.4). In addition, blank gels did not show any appreciable cytotoxicity, whereas 5FU-loaded hydrogels demonstrated efficacy against HCT116 colon cancer cells, which further confirms the potential use of these delivery platforms for direct targeting of 5-FU to the colon.
基于菊粉的水凝胶是结肠靶向系统及其他生物医学应用中药物递送的有用载体。在本项目中，通过氧化菊粉与己二酸二酰肼（AAD）交联制备菊粉水凝胶，无需使用催化剂或引发剂。使用不同技术进一步表征所得水凝胶的物理化学性质，如溶胀实验、体外药物释放、降解和生物相容性测试。通过傅里叶变换红外光谱（FTIR）、热重分析（TGA）和差示扫描量热法（DSC）确认交联。与生理pH值（pH 7.4）相比，在酸性条件（pH 5）下，各种菊粉水凝胶中5-氟尿嘧啶（5FU）的体外释放增强。此外，空白凝胶未显示任何明显的细胞毒性，而负载5FU的水凝胶对HCT116结肠癌细胞显示出疗效，这进一步证实了这些递送平台将5-FU直接靶向结肠的潜在用途。
