植物生长调节剂对哈茨木霉（T8）体外生物防治尖孢镰刀菌的影响。

Effect of plant growth regulators on in vitro biological control of Fusarium oxysporum by Trichoderma harzianum (T8).

作者信息

Badri M, Zamani M R, Motallebi M

机构信息

National Institute for Genetic Engineering and Biotechnology (NIGEB), Tehran, Islamic Republic of Iran.

出版信息

Pak J Biol Sci. 2007 Sep 1;10(17):2850-5. doi: 10.3923/pjbs.2007.2850.2855.

DOI:10.3923/pjbs.2007.2850.2855

PMID:19090187

Abstract

In this study the effect of two plant growth regulators (indolacetic acid, IAA and gibberellic acid, GA3) and also Trichoderma harzianum (T8) on the phytopathogen fungus Fusarium oxysporium (F15) was investigated. IAA and GA3 with 15 and 30 ppm concentration have no significant effect on T. harzianum (T8) growth. The biocontrol activity of T. harzianum on F. oxysporum was slightly decreased by the presence of IAA and/or GA3. Addition of 40 ppm of GA3 to the culture medium of F. oxsporum increased polygalacturonase activity about 100%. A strong increasing effect on chitinase activity (60%) by T. harzianum (T8) was observed in the presence of phytopathogenic fungus F. oxysporum, but 40 ppm IAA and/or GA3 decreased about 47% of chitinase activity of T. harzianum.

摘要

在本研究中，研究了两种植物生长调节剂（吲哚乙酸，IAA和赤霉素，GA3）以及哈茨木霉（T8）对植物病原真菌尖孢镰刀菌（F15）的影响。浓度为15和30 ppm的IAA和GA3对哈茨木霉（T8）的生长没有显著影响。IAA和/或GA3的存在会使哈茨木霉对尖孢镰刀菌的生物防治活性略有降低。向尖孢镰刀菌的培养基中添加40 ppm的GA3可使多聚半乳糖醛酸酶活性提高约100%。在植物病原真菌尖孢镰刀菌存在的情况下，观察到哈茨木霉（T8）对几丁质酶活性有强烈的增强作用（60%），但40 ppm的IAA和/或GA3会使哈茨木霉的几丁质酶活性降低约47%。