State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China.
Shanghai Shuyin Intelligent Technology Co., LTD, Shanghai, China.
Arch Microbiol. 2024 Jul 31;206(8):365. doi: 10.1007/s00203-024-04091-4.
Trichoderma harzianum T4 is a soil fungus that plays an important role in the biological control of plant diseases. The aim of this study was to functionally characterize the β-1,6-glucanase gene Neg1 in T. harzianum T4 and to investigate the effect of its overexpression on biocontrol traits, especially antagonism against pathogenic fungi. We found that overexpression of Neg1 did not affect growth of T. harzianum but enhanced sporulation of T. harzianum T4 cultures. Generally, spores are closely related to the defense ability of defense fungi and can assist their proliferation and improve their colonization ability. Secondly, overexpression of Neg1 also increased the secretion level of various hydrolytic enzymes and enhanced the antagonistic ability against phytopathogenic fungi of Fusarium spp. The results suggest that Neg1 is a key gene for improving the biocontrol effect of T. harzianum T4, which contributes to a better understanding of the mechanism of action of T. harzianum T4 as a fungal biocontrol agent.
哈茨木霉 T4 是一种土壤真菌，在植物病害的生物防治中起着重要作用。本研究旨在对哈茨木霉 T4 中的β-1,6-葡聚糖酶基因 Neg1 进行功能表征，并研究其过表达对生物防治特性的影响，特别是对病原菌的拮抗作用。我们发现，Neg1 的过表达并不影响哈茨木霉的生长，但增强了哈茨木霉 T4 培养物的孢子形成。通常，孢子与防御真菌的防御能力密切相关，能够协助其增殖并提高其定植能力。其次，Neg1 的过表达还增加了各种水解酶的分泌水平，并增强了对尖孢镰刀菌等植物病原菌的拮抗能力。这些结果表明，Neg1 是提高哈茨木霉 T4 生物防治效果的关键基因，有助于更好地理解哈茨木霉 T4 作为真菌生物防治剂的作用机制。
