吡喃糖2-氧化酶（P2O）：在搅拌罐反应器中由云芝生产及其部分特性研究
Pyranose 2-oxidase (P2O): production from trametes versicolor in stirred tank reactor and its partial characterization.
作者信息
Pazarlioglu Nurdan Kasikara, Akkaya Alper, Tahsinsoy Dilek
机构信息
Faculty of Science, Biochemistry Department, Ege University, Bornova-Izmir, Turkiye.
出版信息
Prep Biochem Biotechnol. 2009;39(1):32-45. doi: 10.1080/10826060802589569.
Optimization of pyranose-2-oxidase (P2O) production conditions from Trametes versicolor was carried out in shaking cultures containing glucose, malt, and yeast extracts; the optimum concentration values were found to be 1.5% glucose, 1.0% yeast extract, and 1.0% malt extract, pH 5.0, temperature, 26 degrees C, and agitation rate 150 rpm. For the first time, P2O production was also carried out in a stirred tank reactor (STR) with 2.2 L working volume in the optimized medium composition, and biomass, P2O activity, protein, nitrogen and glucose concentrations were also monitored besides pH and dissolved oxygen (DO). In the STR, P2O activity peaked on day 9. Partial enzyme characterization occurred and optimum pH and temperature were detected as 7.0 and 37 degrees C, respectively. K(m) value was found to be 1.009 mM.
对云芝产吡喃糖-2-氧化酶（P2O）的条件进行了优化，实验在含有葡萄糖、麦芽和酵母提取物的摇瓶培养中进行；发现最佳浓度值为1.5%葡萄糖、1.0%酵母提取物、1.0%麦芽提取物，pH 5.0，温度26℃，搅拌速率150 rpm。首次在工作体积为2.2 L的搅拌罐反应器（STR）中，在优化的培养基组成下进行P2O生产，除了pH和溶解氧（DO）外，还监测了生物量、P2O活性、蛋白质、氮和葡萄糖浓度。在STR中，P2O活性在第9天达到峰值。进行了部分酶学特性分析，检测到最佳pH和温度分别为7.0和37℃。发现K(m)值为1.009 mM。
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