Faculty of Science, Biochemistry Department, Ege University, 35100, Bornova, Izmir, Turkey.
World J Microbiol Biotechnol. 2012 Apr;28(4):1523-31. doi: 10.1007/s11274-011-0955-4. Epub 2011 Nov 26.
The aim of this work was to determine new, different and low-cost substrates that can be used for enzyme production from the white rot fungus Trametes versicolor (ATCC 11235) by taking advantage of the broad substrate specificity of pyranose 2-oxidase. In this report, we investigated the production of pyranose 2-oxidase from T. versicolor (ATCC 11235) using ten different agricultural residues such as clover straw, almond shells, hazelnut cobs, grass and others. Pyranose 2-oxidase activity was determined as 2.332 U/g at the 9th day in a submerged culture containing clover straw and tap water shaken at 150 rpm and 26°C, and the optimum clover straw concentration was determined to be 12 g/l. The effects of different glucose, nitrogen and phosphate sources on the production of pyranose 2-oxidase were studied in the clover straw medium. Analyses of biomass, protein, reduced sugar and nitrogen concentrations were also monitored in a clover straw medium that did not contain carbon or nitrogen and phosphate sources under the parameters determined. The produced pyranose 2-oxidase was used for improving the properties of cotton fabrics.
本工作旨在利用吡喃糖 2-氧化酶广泛的底物特异性，确定新的、不同的和低成本的基质，可用于从白腐菌 Trametes versicolor（ATCC 11235）生产酶。在本报告中，我们研究了使用十种不同的农业废弃物，如三叶草秸秆、杏仁壳、榛子壳、草等，从 T. versicolor（ATCC 11235）生产吡喃糖 2-氧化酶。在含有三叶草秸秆和自来水的浸没培养物中，在 150rpm 和 26°C 下振荡，第 9 天测定吡喃糖 2-氧化酶活性为 2.332 U/g，并且确定了最佳三叶草秸秆浓度为 12 g/l。研究了不同的葡萄糖、氮和磷酸盐源对三叶草秸秆培养基中吡喃糖 2-氧化酶生产的影响。在不含有碳、氮和磷酸盐源的三叶草秸秆培养基中，根据确定的参数，还监测了生物量、蛋白质、还原糖和氮浓度的分析。所产生的吡喃糖 2-氧化酶用于改善棉织物的性能。
