Tsuruoka M, Ishizaki K, Sakurai K, Matsuzaka K, Inoue T
Department of Removable Prosthodontics and Gerodontology, Oral Health Science Center, Tokyo Dental College, Mihama-ku, Chiba, Japan.
J Oral Rehabil. 2008 Dec;35(12):889-97. doi: 10.1111/j.1365-2842.2008.01883.x.
The purpose of this study was to determine the effects of mechanical compression on the palatal mucosa using an experimental palatal base. The palatal base was either pressed onto (stress group) or not pressed onto (fit group) rat palatal mucosa. Blood flow was measured and the animals were sacrificed 6-72 h later for analysis. The expression of heat shock protein 70 (HSP70), vascular endothelial growth factor (VEGF) and proliferation cell nuclear antigen (PCNA) was characterized by immunohistochemical staining. For morphometric analysis, connective tissues were divided into bone side and epithelial side tissues. The ratio of PCNA-positive cells (PCNA score) was calculated, and the expressions of mRNA encoding HSP70 and VEGF was evaluated. Whereas blood flow in the stress group showed ischaemia, none was found in the fit group. Proliferation cell nuclear antigen scores on the bone side were higher than on the epithelial side in the stress group (P < 0.05). Heat shock protein 70- and VEGF-positive cells were observed under compression conditions, particularly in the periosteum. In the stress group, the expressions of mRNA encoding HSP70 and VEGF were highest at 12 h (P < 0.05). These results suggest that mechanical compression of the palatal plate induces ischaemia, and that cells in the underlying denture-supporting tissue, which includes the periosteum, synthesize HSP70 and VEGF to maintain homeostasis under these conditions.
本研究的目的是使用实验性腭基托来确定机械压迫对腭黏膜的影响。将腭基托压在大鼠腭黏膜上（应力组）或不压在其上（贴合组）。测量血流量，并在6 - 72小时后处死动物进行分析。通过免疫组织化学染色来表征热休克蛋白70（HSP70）、血管内皮生长因子（VEGF）和增殖细胞核抗原（PCNA）的表达。对于形态计量分析，将结缔组织分为骨侧和上皮侧组织。计算PCNA阳性细胞的比例（PCNA评分），并评估编码HSP70和VEGF的mRNA的表达。应力组的血流量显示缺血，而贴合组未发现缺血情况。应力组中骨侧的增殖细胞核抗原评分高于上皮侧（P < 0.05）。在压迫条件下观察到HSP70和VEGF阳性细胞，特别是在骨膜中。在应力组中，编码HSP70和VEGF的mRNA的表达在12小时时最高（P < 0.05）。这些结果表明，腭板的机械压迫会诱导缺血，并且在这些条件下，包括骨膜在内的下方义齿支持组织中的细胞会合成HSP70和VEGF以维持体内稳态。
