Department of Anatomy, Tokyo Medical University.
Eur J Histochem. 2020 Feb 26;64(1):3095. doi: 10.4081/ejh.2020.3095.
Angiogenesis is an important issue related to normal growth and differentiation, and it is a critical issue in the progression of human disease in oral mucosa. Tooth marks occur after clenching the teeth for a long period under muscle tension in the human oral cavity. However, the sites of angiogenesis, cell differentiation and microvessel density are not known for human mucosa with tooth marks. Therefore, we investigated the relationship between the markers of differentiation (Ki-67), angiogenesis (CD31, D2-40, VEGF-A), and marks from teeth in the second molar region using immunohistochemical methods. In addition, we compared these areas with the mucous membrane. Our results revealed blood and lymphoid vessels in irregular mucosa structures, and the vessels in the oral mucosa were observed in three types of samples: dentulous, denture attachment (containing partial teeth), and edentulous samples. The localization of the angiogenesis was related to the structure of the oral mucosa of connective tissue in humans, such as the mucosal fold-like of the buccal region. Using principal component analysis (PCA), we found that tooth occlusal condition, gender, anti-VEGF-A reaction levels in oral mucosa of the epithelium were positive factors in all groups, which is in contrast to the negative association of Ki-67 reaction in the epithelium and CD31 expression. In addition, Ki-67 reaction in oral mucosa had negative impacts, in contrast to the positive association of D2-40. These PCA properties provide useful information for future study of tumour progression or mechanical stress in remodelling of oral mucosa and angiogenesis. Moreover, mechanical stress of the occlusal condition may be correlated with tumour angiogenic activity and cell differentiation in human oral mucosa.
血管生成是与正常生长和分化相关的重要问题，也是口腔黏膜人类疾病进展的关键问题。在人类口腔中，由于肌肉张力的作用，长时间咬牙会导致齿痕的产生。然而，对于有齿痕的人类黏膜，其血管生成、细胞分化和微血管密度的部位尚不清楚。因此，我们采用免疫组织化学方法研究了第二磨牙区域牙齿标记物与分化标志物（Ki-67）、血管生成标志物（CD31、D2-40、VEGF-A）之间的关系。此外，我们还将这些区域与黏膜进行了比较。我们的结果显示，不规则黏膜结构中有血液和淋巴血管，在有牙、义齿附着（含部分牙齿）和无牙三种类型的样本中均可观察到口腔黏膜中的血管。血管生成的定位与人类结缔组织黏膜的结构有关，如颊部的黏膜褶皱样结构。通过主成分分析（PCA），我们发现所有组中，牙咬合状态、性别、口腔黏膜上皮的抗 VEGF-A 反应水平是阳性因素，而上皮 Ki-67 反应和 CD31 表达呈负相关。此外，口腔黏膜 Ki-67 反应呈阴性，而与 D2-40 呈正相关。这些 PCA 特性为未来研究口腔黏膜肿瘤进展或机械应力重塑和血管生成提供了有用的信息。此外，咬合状态的机械应力可能与人类口腔黏膜的肿瘤血管生成活性和细胞分化有关。
