Purification and properties of rat brain succinic semialdehyde dehydrogenase.
作者信息
Cash C, Ciesielski L, Maitre M, Mandel P
出版信息
Biochimie. 1977;59(3):257-68. doi: 10.1016/s0300-9084(77)80142-9.
PMID:19091
Abstract
Succinic semialdehyde dehydrogenase from rat brain has been purified to electrophoretic homogeneity. It has a molecular weight of about 140, 000 and is composed of two apparently identical subunits. The reaction catalized by the pure protein is entirely dependent on endogenous --SH groups. The Kim (limits) for NAD and succinic semialdehyde are 2 X 10(-5) M and 1 X 10(-4) M respectively at the optimum pH of 8.6. Inhibition studies show that the reaction mechanism is a compulsory ordered on where NAD binds first followed by succinic semialdehyde.
摘要
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