Makino Nobuko, Ookawara Shigeo, Katoh Kazuo, Ohta Yasushi, Ichikawa Masumi, Ichimura Keiichi
Department of Otolaryngology-Head and Neck Surgery, Jichi Medical University School of Medicine, 3311-1 Yakushiji, Shimotsuke, Tochigi 329-0498, Japan.
Chem Senses. 2009 Feb;34(2):171-9. doi: 10.1093/chemse/bjn074. Epub 2008 Dec 16.
Transmission electron microscopy was used to study the responses of the supporting cells of the olfactory epithelium at 1-5 days after surgical ablation of the olfactory bulb (bulbectomy). In intact olfactory epithelium, lamellar smooth endoplasmic reticulum and rod-shaped mitochondria were distinctly observed in the supporting cells. On the first day after bulbectomy, bending of the microvilli and an increase in the smooth endoplasmic reticulum were observed. Cristae of the mitochondria became obscure, and the density of the mitochondrial matrix decreased. On the second day after bulbectomy, the number of microvilli decreased, broad cytoplasmic projections that contained cytoplasmic organelles protruded into the luminal side, and the mitochondria were swollen. On the fifth day after bulbectomy, microvilli seemed to be normal and some cells had large cytoplasmic projections that protruded toward the lumen of the nasal cavity. Within the cytoplasmic projections of the supporting cells, a large lamellar and reticular-shaped smooth endoplasmic reticulum was evident. Mitochondria exhibited almost normal morphology. The current findings demonstrate that morphological changes occur in the supporting cells after bulbectomy. This new evidence hypothesizes that these changes represent events that contribute to the regeneration of the olfactory epithelium after bulbectomy.
利用透射电子显微镜研究了嗅球切除术后1至5天嗅上皮支持细胞的反应。在完整的嗅上皮中，支持细胞内可清晰观察到板层状滑面内质网和杆状线粒体。嗅球切除术后第一天，观察到微绒毛弯曲且滑面内质网增加。线粒体嵴变得模糊，线粒体基质密度降低。嗅球切除术后第二天，微绒毛数量减少，含有细胞质细胞器的宽阔细胞质突起向管腔侧突出，线粒体肿胀。嗅球切除术后第五天，微绒毛似乎正常，一些细胞有朝向鼻腔管腔突出的大细胞质突起。在支持细胞的细胞质突起内，明显可见大的板层状和网状滑面内质网。线粒体表现出几乎正常的形态。目前的研究结果表明，嗅球切除术后支持细胞会发生形态变化。这一新证据推测，这些变化代表了有助于嗅球切除术后嗅上皮再生的事件。
