Pope R M, Landay A, Modlin R L, Lessard J, Koch A E
Department of Medicine, Northwestern University Medical School, Chicago, Illinois.
Cell Immunol. 1991 Oct 1;137(1):127-38. doi: 10.1016/0008-8749(91)90063-h.
In patients with inflammatory synovitis, the proliferative response by lymphocytes from synovial fluid to soluble mycobacterial antigens is enhanced relative to those from peripheral blood. Earlier studies suggested that gamma/delta T cell receptor positive (TCR+) T lymphocytes may significantly contribute to the mycobacterial-specific synovial fluid response. We therefore examined the relationship of the T cell proliferative response to Mycobacterium tuberculosis antigens and the presence of gamma/delta TCR+ T cells employing several monoclonal antibodies. No consistent increase of gamma/delta TCR+ T cells was noted in inflammatory synovial fluids or tissues. Nonetheless, lymphocytes from the majority of the synovial fluids proliferated vigorously in response to water-soluble M. tuberculosis antigens. There was no relationship between the percentage of gamma/delta TCR+ T lymphocytes and the intensity of the proliferative response. In contrast, stimulation with whole mycobacterial organisms was capable of enriching the gamma/delta TCR+ cell population obtained from the peripheral blood of tuberculosis skin test positive normal controls and from some inflammatory synovial fluids. These observations do not support a role for mycobacteria reactive gamma/delta TCR+ synovial T lymphocytes in response to soluble mycobacterial antigens or in the local pathogenesis of inflammatory synovitis.
在炎性滑膜炎患者中，相对于外周血淋巴细胞，滑膜液淋巴细胞对可溶性分枝杆菌抗原的增殖反应增强。早期研究表明，γ/δ T细胞受体阳性（TCR+）T淋巴细胞可能对分枝杆菌特异性滑膜液反应有显著贡献。因此，我们使用几种单克隆抗体研究了T细胞对结核分枝杆菌抗原的增殖反应与γ/δ TCR+ T细胞存在之间的关系。在炎性滑膜液或组织中未观察到γ/δ TCR+ T细胞持续增加。尽管如此，大多数滑膜液中的淋巴细胞对水溶性结核分枝杆菌抗原反应强烈增殖。γ/δ TCR+ T淋巴细胞百分比与增殖反应强度之间没有关系。相反，用完整分枝杆菌刺激能够富集从结核菌素皮肤试验阳性正常对照的外周血以及一些炎性滑膜液中获得的γ/δ TCR+细胞群体。这些观察结果不支持分枝杆菌反应性γ/δ TCR+滑膜T淋巴细胞在对可溶性分枝杆菌抗原反应或炎性滑膜炎局部发病机制中的作用。
