Kjeldsen-Kragh J, Quayle A, Kalvenes C, Førre O, Sørskaar D, Vinje O, Thoen J, Natvig J B
Institute of Immunology and Rheumatology, Oslo Sanitetsforenings Rheumatism Hospital, Norway.
Scand J Immunol. 1990 Dec;32(6):651-9. doi: 10.1111/j.1365-3083.1990.tb03207.x.
Using the anti-TcR gamma/delta-1 monoclonal antibody and flow cytometry, we examined the number of T gamma delta cells in paired samples of peripheral blood and synovial fluid or tissue from 24 children with juvenile rheumatoid arthritis (JRA), five adult patients with JRA, and 14 patients with rheumatoid arthritis (RA). No significant difference was found in the synovial compartment T gamma delta values compared with the blood in JRA, adult JRA, or RA patients. Nor was any significant difference found in the peripheral blood or synovial compartment T gamma delta values in any of the three patient groups compared with the peripheral blood of normal controls. However, seven of the children with JRA had very high T gamma delta values in the synovial compartment while none of the normal children had high T gamma delta values in the blood (P = 0.02, Fisher's exact test). This may indicate a possible separate JRA patient group with high T gamma delta levels in the synovial compartment. In six JRA patients further analysed for T gamma delta subpopulations, a significant predominance of V delta 1+ cells was found in the synovial compartment compared with the corresponding peripheral blood samples (P less than 0.05, Wilcoxon's signed test) and with peripheral blood of child controls (P less than 0.05, Mann-Whitney U test). In these six patients, the T gamma delta-cell expression of the very early activation antigen CD69 were significantly higher (P less than 0.05, Wilcoxon's signed test) in the synovial compartment compared with the peripheral blood. Synovial T gamma delta cells expressing HLA-DR and interleukin 2 receptors could also be detected, in contrast to the peripheral blood in which no T gamma delta cells expressing these antigens could be found. These data suggest that the synovial T gamma delta cells had been activated in vivo.
我们使用抗T细胞受体γ/δ-1单克隆抗体和流式细胞术，检测了24例幼年类风湿性关节炎（JRA）患儿、5例成年JRA患者以及14例类风湿性关节炎（RA）患者外周血与滑膜液或组织配对样本中的Tγδ细胞数量。在JRA、成年JRA或RA患者中，滑膜腔Tγδ值与血液相比未发现显著差异。与正常对照者的外周血相比，这三组患者的外周血或滑膜腔Tγδ值也未发现显著差异。然而，7例JRA患儿滑膜腔中的Tγδ值非常高，而正常儿童血液中没有高Tγδ值的情况（P = 0.02，Fisher精确检验）。这可能表明存在一个滑膜腔Tγδ水平高的JRA患者亚组。在进一步分析Tγδ亚群的6例JRA患者中，与相应外周血样本相比（P<0.05，Wilcoxon符号秩检验）以及与儿童对照者的外周血相比（P<0.05，Mann-Whitney U检验），滑膜腔中Vδ1+细胞显著占优势。在这6例患者中，与外周血相比，滑膜腔中极早期活化抗原CD69的Tγδ细胞表达显著更高（P<0.05，Wilcoxon符号秩检验）。与外周血中未发现表达这些抗原的Tγδ细胞相反，滑膜中也能检测到表达HLA-DR和白细胞介素2受体的Tγδ细胞。这些数据表明滑膜Tγδ细胞在体内已被激活。
