Sadouk M B, Rajzbaum G, Vaquero C, Amor B, Toubert A
INSERM U. 283, Hôpital Cochin, Paris, France.
Cell Immunol. 1991 Oct 1;137(1):239-44. doi: 10.1016/0008-8749(91)90073-k.
Circulating T lymphocytes from patients with rheumatoid arthritis (RA) were found to produce low concentrations of interferon-gamma (IFN-gamma) and express low amounts of this messenger RNA (mRNA) in response to stimulation with phytohemagglutinin (PHA). In this study, we have examined the superinduction effect of cycloheximide (CHX) on the IFN-gamma mRNA expression in PHA-stimulated T lymphocytes from nine patients with active RA compared to three healthy individuals. When CHX was added 6 hr postactivation, the expression of IFN-gamma mRNA in RA patients increased significantly at 24 and 48 hr (P less than 0.01) and reached levels similar to those observed in controls. We suggest that posttranscriptional events could play a role in the defective production of IFN-gamma observed in RA.
类风湿性关节炎（RA）患者的循环T淋巴细胞在受到植物血凝素（PHA）刺激后，被发现产生低浓度的γ-干扰素（IFN-γ）并表达少量的该信使核糖核酸（mRNA）。在本研究中，我们检测了环己酰亚胺（CHX）对9例活动期RA患者与3名健康个体PHA刺激的T淋巴细胞中IFN-γ mRNA表达的超诱导作用。当在激活后6小时添加CHX时，RA患者中IFN-γ mRNA的表达在24和48小时显著增加（P<0.01），并达到与对照组相似的水平。我们认为转录后事件可能在RA中观察到的IFN-γ产生缺陷中起作用。
