Sadouk M, Vaquero C, de la Tour B, Amor B, Toubert A
INSERM U. 283, Paris, France.
Clin Immunol Immunopathol. 1990 Jul;56(1):37-45. doi: 10.1016/0090-1229(90)90167-o.
A decreased production of interferon-gamma (IFN-gamma) and interleukin-2 (IL-2) upon in vitro stimulation by phytohemagglutinin (PHA) peripheral blood leucocyte in active rheumatoid arthritis (RA) patients has been described as an important immunological abnormality in this disease. However, the molecular level at which this defect might occur has not been fully documented. We have investigated the kinetics of IFN-gamma, interleukin-2 receptor (IL-2 R), and interleukin-1 beta (IL-1 beta) messenger RNA (mRNA) expression in RA patients (n = 9) and normal controls (n = 5) after PHA leucocyte activation. We demonstrate here a significantly decreased expression of IFN-gamma mRNA in RA patients without modification of its kinetics associated with a similar IL-1 beta mRNA expression.
有研究称，在活性类风湿关节炎（RA）患者中，经植物血凝素（PHA）体外刺激外周血白细胞后，γ干扰素（IFN-γ）和白细胞介素-2（IL-2）的产生减少，这被视为该疾病一项重要的免疫异常。然而，这种缺陷可能出现的分子水平尚未得到充分记录。我们研究了RA患者（n = 9）和正常对照者（n = 5）在PHA白细胞激活后，IFN-γ、白细胞介素-2受体（IL-2 R）和白细胞介素-1β（IL-1β）信使核糖核酸（mRNA）表达的动力学情况。我们在此证明，RA患者中IFN-γ mRNA的表达显著降低，其动力学未发生改变，且IL-1β mRNA表达情况与之相似。
