Hamberger A, Nyström B, Larsson S, Silfvenius H, Nordborg C
Institute of Neurobiology, University of Gothenburg, Sweden.
Epilepsy Res. 1991 May-Jun;9(1):32-43. doi: 10.1016/0920-1211(91)90044-g.
Extracellular fluid was topically sampled with a dialysis probe during electrocorticography from the exposed cerebral cortex in 23 patients undergoing epilepsy surgery. Sampling was done in parallel from epileptiform regions and from non-epileptic areas. The former were classified according to the histopathology, into neoplastic, non-tumoral or 'special cases'. The epileptiform regions had significantly higher extracellular concentrations of alanine, glycine and phosphoethanolamine in the majority of the cases. The excised epileptic lesions were analyzed to provide the corresponding intracellular concentrations of amino acids. Several of the non-tumoral group showed high concentrations of GABA, ethanolamine and alanine. The intra- to extracellular concentration ratio for amino acids was low for phosphoethanolamine, glycine, serine and glutamine in most of the samples of epileptiform cortex, while the intracellular accumulative ability for ethanolamine apparently was stronger in epileptiform than in normal cortex.
在23例接受癫痫手术的患者中，在大脑皮层电图监测期间，用透析探针从暴露的大脑皮层局部采集细胞外液样本。同时从癫痫样区域和非癫痫区域进行采样。前者根据组织病理学分类为肿瘤性、非肿瘤性或“特殊病例”。在大多数情况下，癫痫样区域的细胞外丙氨酸、甘氨酸和磷酸乙醇胺浓度显著更高。对切除的癫痫病灶进行分析，以提供相应的细胞内氨基酸浓度。非肿瘤组中的几例显示出高浓度的γ-氨基丁酸、乙醇胺和丙氨酸。在大多数癫痫样皮层样本中，磷酸乙醇胺、甘氨酸、丝氨酸和谷氨酰胺的细胞内与细胞外浓度比很低，而癫痫样皮层中乙醇胺的细胞内蓄积能力明显强于正常皮层。
