Prelog Martina, Schwarzenbrunner Nora, Tengg Elisabeth, Sailer-Höck Michaela, Kern Hannelore, Zimmerhackl Lothar Bernd, Brunner Juergen
Department of Pediatrics, Pediatrics I, Medical University Innsbruck, Anichstr. 35, 6020 Innsbruck, Austria.
Clin Rheumatol. 2009 Apr;28(4):385-9. doi: 10.1007/s10067-008-1057-z. Epub 2008 Dec 18.
The study was aimed to investigate whether quantities of CD8(+) T cell subsets are normal in juvenile idiopathic arthritis (JIA) patients with disease remission compared to age-matched healthy donors (HD) and whether chronological age may have an impact on proportions of naive CD8(+) T cells. CD8(+) T cell subsets were analyzed in 17 JIA patients and 32 age-matched HD by flow cytometry. JIA patients showed lower CD3(+)CD8(+) T cells compared to HD. Total counts of CD8(+)CD28(+) and CD8(+)CD28(+)CD45RA(+) T cells were inversely correlated to chronological age in JIA patients and HD. In JIA patients, percentages of CD8(+)CD28(+)CD45RA(+) T cells and of CD62L-expressing CD8(+)CD28(+)CD45RA(+) T cells showed a negative correlation with age. The trend to lower CD28(+)CD45RA(+) T cell proportions in aged JIA patients in remission may reflect a disturbed T cell homeostasis independently of disease activity and may be due to an intrinsic effect in reconstitution of the peripheral T cells.
本研究旨在调查与年龄匹配的健康供者（HD）相比，疾病缓解的幼年特发性关节炎（JIA）患者中CD8(+) T细胞亚群数量是否正常，以及实足年龄是否可能对初始CD8(+) T细胞比例产生影响。通过流式细胞术分析了17例JIA患者和32例年龄匹配的HD中的CD8(+) T细胞亚群。与HD相比，JIA患者的CD3(+)CD8(+) T细胞较低。JIA患者和HD中CD8(+)CD28(+)和CD8(+)CD28(+)CD45RA(+) T细胞的总数与实足年龄呈负相关。在JIA患者中，CD8(+)CD28(+)CD45RA(+) T细胞百分比以及表达CD62L的CD8(+)CD28(+)CD45RA(+) T细胞百分比与年龄呈负相关。缓解期老年JIA患者中CD28(+)CD45RA(+) T细胞比例降低的趋势可能反映了独立于疾病活动的T细胞内环境稳定紊乱，可能是由于外周T细胞重建中的内在效应。
