Chiesa Sabrina, Prigione Ignazia, Morandi Fabio, Buoncompagni Antonella, Picco Paolo, Bocca Paola, Martini Alberto, Pistoia Vito, Gattorno Marco
2nd Division of Pediatrics, "G. Gaslini" Scientific Institute for Children, Genoa, Italy.
J Rheumatol. 2004 Oct;31(10):2048-54.
It has been suggested that CD45RO+CD27+ T cells represent recently activated memory cells, whereas CD45RO+CD27- T cells are activated memory T cells in the process of differentiating into effector cells. We investigated (1) CCR7 and CCR5 expression and (2) modulation of cytokine expression in "early" (CD27+) and "differentiated" (CD27-) memory CD4+ T cells from peripheral blood and synovial fluid (SF) of patients with juvenile idiopathic arthritis (JIA).
SF CD4+CD45RO+CD27+ and CD27- memory T cells from 6 patients with JIA were tested by flow cytometry for intracellular interferon-gamma (IFN-gamma) and interleukin 4 (IL-4) after in vitro priming with CD3 and CD28 mAb in the presence of IL-4, and subsequent culture with IL-2.
SF CD4+CD45RO+CD27+ cells contained higher proportions of CCR7+ (median 46% vs 23%) and lower proportions of CCR5+ (73% vs 90%) cells than paired CD27- T cells. Both CD27+ and CD27- memory T helper cells from SF displayed a higher IFN-gamma/IL-4 ratio than their peripheral blood counterparts. No significant difference was observed in the percentage of IFN-gamma-expressing cells between CD27+ (32%, range 4-47%) and CD27- (29.4%, range 5-52%) memory T helper cells from SF.
Irrespective of their differentiation stage, both CD27+ and CD27- SF memory T helper cells were found to switch from a proinflammatory to an antiinflammatory pattern of cytokine production.
有人提出，CD45RO+CD27+ T细胞代表近期活化的记忆细胞，而CD45RO+CD27- T细胞是在分化为效应细胞过程中的活化记忆T细胞。我们研究了（1）幼年特发性关节炎（JIA）患者外周血和滑液（SF）中“早期”（CD27+）和“分化”（CD27-）记忆CD4+ T细胞中CCR7和CCR5的表达，以及（2）细胞因子表达的调节情况。
对6例JIA患者的SF CD4+CD45RO+CD27+和CD27-记忆T细胞进行流式细胞术检测，在IL-4存在的情况下用CD3和CD28单克隆抗体进行体外刺激，随后用IL-2培养，检测细胞内干扰素-γ（IFN-γ）和白细胞介素4（IL-4）。
与配对的CD27- T细胞相比，SF CD4+CD45RO+CD27+细胞中CCR7+细胞比例更高（中位数46%对23%），CCR5+细胞比例更低（73%对90%）。SF中的CD27+和CD27-记忆辅助性T细胞的IFN-γ/IL-4比值均高于外周血中的对应细胞。SF中CD27+（32%，范围4-47%）和CD27-（29.4%，范围5-52%）记忆辅助性T细胞中表达IFN-γ的细胞百分比无显著差异。
无论其分化阶段如何，SF中的CD27+和CD27-记忆辅助性T细胞均从促炎细胞因子产生模式转变为抗炎模式。
