Panahandeh J, Valizadeh M, Khosroshahly M, Yermishin A, Khoei F R
Department of Horticultural Sciences, Faculty of Agriculture, University of Tabriz, Iran.
Pak J Biol Sci. 2007 Jun 15;10(12):2002-7. doi: 10.3923/pjbs.2007.2002.2007.
The cultivated potato Solanum tuberosum L. is an autotetraploid with Endosperm Balance Number (EBN) of 4. There are some allotetraploid 2EBN wild species with desirable traits for potato improvement. However, it is very difficult to cross between these two groups due to differences in EBN. Eleven genotypes of autotetraploid cultivated potato including the six cultivars of subsp. Tuberosum (tbr) and five clones of subsp. Andigena (adg) were crossed with two wild allotetraploid species: S. acaule (acl) and S. stoloniferum (sto). For asses the pollen-pistil incompatibility, some ofpollinated flowers were fixed in Carnoy's solution and stained with aniline blue. Evaluation with fluorescent microscope revealed that pre-zygotic pollen-pistil incompatibility in these crosses is frequent. The pistils of both subsp. of cultivated potato were incompatible with pollens of acl but when sto used as staminate parent it was compatible with five cultivars of subsp. Tuberosum, but incompatible with most ofsubsp. Andigena (4 clones). From 739, 4x, 4EBN x 4x, 2EBN crosses we couldn't get any viable seed. In reciprocal crosses, allotetraploid species were pollinated with a mix of pollens from Tuberosum or Andigena clones. The crosses of sto x adg, acl x adg and acl x tbr were compatible but in the case of sto x tbr the situation was not clear. From these crosses 157 fruits were obtained but most of them contained only shrink seed and we find just one hybrid from the sto x tbr combination. The hybrid was tetraploid (2n = 4x = 48) and vigorous with profuse flowering and good fertility. When this hybrid was used as the pistillate parent it was compatible with both parental species.
栽培马铃薯（Solanum tuberosum L.）是一种胚乳平衡数（EBN）为4的同源四倍体。有一些具有2EBN的异源四倍体野生种，它们具有可用于改良马铃薯的优良性状。然而，由于EBN的差异，这两组之间很难杂交。11种基因型的同源四倍体栽培马铃薯，包括6个亚种Tuberosum（tbr）的品种和5个亚种Andigena（adg）的无性系，与两个野生异源四倍体物种进行了杂交：S. acaule（acl）和S. stoloniferum（sto）。为了评估花粉-雌蕊不亲和性，将一些授粉后的花朵固定在卡诺氏液中，并用苯胺蓝染色。荧光显微镜评估显示，这些杂交中合子前的花粉-雌蕊不亲和性很常见。栽培马铃薯两个亚种的雌蕊与acl的花粉不亲和，但当以sto作为雄亲本时，它与5个亚种Tuberosum的品种亲和，但与大多数亚种Andigena（4个无性系）不亲和。在739个4x、4EBN×4x、2EBN的杂交组合中，我们没有得到任何有活力的种子。在正反交中，用来自Tuberosum或Andigena无性系的混合花粉给异源四倍体物种授粉。sto×adg、acl×adg和acl×tbr的杂交组合是亲和的，但在sto×tbr的情况下情况不明确。从这些杂交组合中获得了157个果实，但大多数果实中只含有皱缩的种子，我们仅从sto×tbr组合中发现了一个杂种。该杂种是四倍体（2n = 4x = 48），生长旺盛，开花繁茂且育性良好。当这个杂种用作雌亲本时，它与两个亲本物种都亲和。
