Chamani-Tabriz Leili, Tehrani Mahmood J, Akhondi Mohammad Mehdi A, Mosavi-Jarrahi Alireza, Zeraati Hojjat, Ghasemi Jamileh, Asgari Soheila, Kokab Abasali, Eley Adrian R
Department of Reproductive Infections, Reproductive Biotechnology Research Center, Avesina Research Institute, ACECR, P.O. Box 19835-177, Tehran, Iran.
Pak J Biol Sci. 2007 Dec 15;10(24):4490-4. doi: 10.3923/pjbs.2007.4490.4494.
This study was designed to estimate the prevalence of Chlamydia infection in women attending Obstetrics and Gynaecology clinics in Tehran, during May 2003 to October 2003. Women attending Obstetrics and Gynaecology clinics aged 15-42 were recruited by Sequential Random Sampling. Those who had not passed urine in the last hour were eligible. Informed consent was obtained and a questionnaire completed after being interviewed by a midwife. First void urine was collected and after DNA extraction from urine specimen, PCR tests were performed; urine DNA samples were tested by strand displacement amplification (SDA) for Chlamydia confirmation. 12.6% (133/1052) tested positive for Chlamydia by PCR. Of these PCR positive samples, 86 were available for re-testing by SDA and 67 were positive giving a correlation between the tests of 78%. This gave an overall true prevalence of 6.4% which is however, underestimated. No statistical differences were seen between patient age groups, details of personal and reproductive history and combined PCR and SDA positivity for C. trachomatis. A 12.6% prevalence of Chlamydia trachomatis was found by PCR testing which is cost effective to screen and treat. Despite limitations in re-testing PCR-positive samples by SDA, a 78% correlation between tests confirms a high prevalence of C. trachomatis. Non-invasive screening of women was therefore a success in this group of patients. As this was the first time that more sensitive molecular methods were used for detection of C. trachomatis, prevalence in such a big sample size, the results are considerable. However, we suggest further such testing.
本研究旨在估算2003年5月至2003年10月期间在德黑兰妇产科诊所就诊的女性衣原体感染率。采用序贯随机抽样法招募年龄在15 - 42岁之间、前往妇产科诊所就诊的女性。过去一小时内未排尿者符合入选条件。获得知情同意后，由助产士进行访谈并完成一份问卷。收集首次晨尿，从尿液标本中提取DNA后进行PCR检测；尿液DNA样本通过链置换扩增（SDA）进行检测以确认衣原体感染。PCR检测显示12.6%（133/1052）的患者衣原体呈阳性。在这些PCR阳性样本中，86份可用于SDA重新检测，其中67份呈阳性，两种检测方法的相关性为78%。总体真实感染率为6.4%，但该结果被低估。在患者年龄组、个人及生殖史细节以及沙眼衣原体PCR和SDA联合阳性率方面未发现统计学差异。通过PCR检测发现沙眼衣原体感染率为12.6%，这种筛查和治疗方法具有成本效益。尽管用SDA对PCR阳性样本进行重新检测存在局限性，但两种检测方法78%的相关性证实了沙眼衣原体的高感染率。因此，对女性进行非侵入性筛查在这组患者中取得了成功。由于这是首次使用更灵敏的分子方法检测沙眼衣原体，且样本量如此之大，结果相当可观。然而，我们建议进一步开展此类检测。
