Salamonsen L A, Hampton A L, Clements J A, Findlay J K
Prince Henry's Institute of Medical Research, South Melbourne, Vic., Australia.
J Reprod Fertil. 1991 Jul;92(2):393-406. doi: 10.1530/jrf.0.0920393.
Expression of the gene for prostaglandin synthase (PGS) was examined in whole endometrial tissue derived from ewes during the oestrous cycle (Days 4-14), on Day 15 of pregnancy and following ovariectomy and treatment with ovarian steroid hormones. Whilst no significant differences were seen in PGS mRNA concentrations analysed by Northern blot analysis in endometrial tissue during the oestrous cycle or in early pregnancy, treatment of ovariectomized (OVX) ewes with oestradiol-17 beta markedly reduced endometrial PGS mRNA concentration. There was no difference in PGS mRNA concentration in ewes treated with progesterone, either alone or in conjunction with oestrogen, from that in OVX controls. In contrast, differences in immunolocalization of PGS observed in uterine tissue from OVX-steroid-treated ewes were much more marked and reflected similar changes seen previously in the immunocytochemical distribution of endometrial PGS during the oestrous cycle. In OVX ewes and those treated with oestrogen, immunocytochemical staining for PGS was seen in stromal cells, but little immunoreactive PGS was located in the endometrial epithelial cells. However, in ewes treated with progesterone alone or with oestrogen plus progesterone, PGS was found in luminal and glandular epithelial cells and in stromal cells. Intensity of immunostaining for PGS in endothelial cells and myometrium did not differ between the treatments. Thus, whilst oestrogen lowers PGS mRNA in the endometrium, presumably in stroma, it may also increase the stability of the enzyme itself in the stromal cells. Although oestradiol-17 beta has no effect on PGS in endometrial epithelium, progesterone stimulates the production of PGS in endometrial epithelial cells without altering the overall abundance of PGS mRNA in the endometrium as a whole. Conceptus-induced changes in PGF-2 alpha release by ovine endometrium would not appear to be mediated via effects on PGS gene expression or protein synthesis.
在发情周期（第4 - 14天）、妊娠第15天以及去卵巢并用卵巢甾体激素处理后的母羊的整个子宫内膜组织中，检测了前列腺素合酶（PGS）基因的表达。在发情周期或妊娠早期，通过Northern印迹分析子宫内膜组织中PGS mRNA浓度未发现显著差异，但用17β - 雌二醇处理去卵巢（OVX）母羊可显著降低子宫内膜PGS mRNA浓度。单独使用孕酮或与雌激素联合使用的母羊，其PGS mRNA浓度与OVX对照组无差异。相比之下，在去卵巢并用甾体激素处理的母羊子宫组织中观察到的PGS免疫定位差异更为明显，反映了发情周期中子宫内膜PGS免疫细胞化学分布中先前观察到的类似变化。在OVX母羊和用雌激素处理的母羊中，PGS免疫细胞化学染色见于基质细胞，但子宫内膜上皮细胞中几乎没有免疫反应性PGS。然而，在单独用孕酮或用雌激素加孕酮处理的母羊中，PGS见于腔上皮和腺上皮细胞以及基质细胞。各处理组内皮细胞和子宫肌层中PGS免疫染色强度无差异。因此，虽然雌激素降低了子宫内膜中PGS mRNA的水平，推测是在基质中，但它也可能增加了基质细胞中该酶本身的稳定性。虽然17β - 雌二醇对子宫内膜上皮中的PGS没有影响，但孕酮可刺激子宫内膜上皮细胞中PGS的产生，而不改变整个子宫内膜中PGS mRNA的总体丰度。绵羊子宫内膜中由孕体诱导的PGF - 2α释放变化似乎不是通过对PGS基因表达或蛋白质合成的影响来介导的。
