Clinic of Pediatric Surgery, Orthopedics and Traumatology, the Faculty Hospital Brno, Brno, Czech Republic.
Physiol Res. 2009;58(6):885-893. doi: 10.33549/physiolres.931669. Epub 2008 Dec 17.
This study appears from an experiment previously carried out in New Zealand white rabbits. Allogenic mesenchymal stem cells (MSCs) were transplanted into an iatrogenically-created defect in the lateral section of the distal physis of the left femur in 10 miniature pigs. The right femur with the same defect served as a control. To transfer MSCs, a freshly prepared porous scaffold was used, based on collagen and chitosan, constituting a compact tube into which MSCs were implanted. The pigs were euthanized four months after the transplantation. On average, the left femur with transplanted MSCs grew more in length (0.56+/-0.14 cm) compared with right femurs with physeal defect without transplanted MSCs (0.14+/-0.3 cm). The average angular (valgus) deformity of the left femur had an angle point of 0.78 degrees , following measurement and X-ray examination, whereas in the right femur without transplantation it was 3.7 degrees. The initial results indicate that preventive transplantation of MSCs into a physeal defect may prevent valgus deformity formation and probably also reduce disorders of the longitudinal bone growth. This part of our experiment is significant in the effort to advance MSCs application in human medicine by using pig as a model, which is the next step after experimenting on rabbits.
本研究源自先前在新西兰大白兔身上进行的一项实验。同种异体间充质干细胞（MSCs）被移植到 10 头小型猪左侧股骨远端干骺侧的医源性缺损处。右侧具有相同缺损的股骨作为对照。为了移植 MSCs，使用了一种新制备的基于胶原蛋白和壳聚糖的多孔支架，构成了一个紧密的管腔，将 MSCs 植入其中。移植后四个月，猪被安乐死。平均而言，与未移植 MSCs 的右侧具有骺板缺损的股骨相比，移植 MSCs 的左侧股骨在长度上生长更多（0.56+/-0.14 厘米）。经过测量和 X 射线检查，左侧股骨的平均（外翻）畸形角度为 0.78 度，而在未移植的右侧股骨中为 3.7 度。初步结果表明，预防性将 MSCs 移植到骺板缺损处可能预防外翻畸形的形成，并且可能还减少了骨纵向生长的紊乱。我们实验的这一部分对于使用猪作为模型推进 MSCs 在人类医学中的应用具有重要意义，这是在兔子实验之后的下一步。
