Seppen Jurgen, Filali Ebtisam El, Elferink Ronald Oude
AMC Liver Center, Amsterdam, The Netherlands.
Methods Mol Biol. 2009;481:75-82. doi: 10.1007/978-1-59745-201-4_7.
In this chapter, we describe techniques used to determine the efficiency of hepatocyte transplantation in animal models of liver disease. We have included the Gunn rat as a model of an inherited liver disease without hepatocyte damage and Abcb4 knockout mice as a model for an inherited liver disease with hepatocyte damage. Immunodeficient mice are included as an animal model for human hepatocyte transplantation.We describe problems that can be encountered in the maintenance and breeding of Gunn rats and immunodeficient Rag2/gamma common knockout mice. Protocols for the collection of bile in rats and mice are described, and we have also detailed the detection of green fluorescent protein (GFP)-labelled human hepatocytes in immunodeficient mice in this chapter.
在本章中，我们描述了用于确定肝细胞移植在肝病动物模型中效率的技术。我们纳入了冈恩大鼠作为无肝细胞损伤的遗传性肝病模型，以及Abcb4基因敲除小鼠作为有肝细胞损伤的遗传性肝病模型。免疫缺陷小鼠被用作人类肝细胞移植的动物模型。我们描述了在饲养和繁殖冈恩大鼠及免疫缺陷Rag2/γ共同基因敲除小鼠时可能遇到的问题。描述了大鼠和小鼠胆汁收集的方案，并且在本章中我们还详细介绍了免疫缺陷小鼠中绿色荧光蛋白（GFP）标记的人类肝细胞的检测。
