Safinia Niloufar, Minger Stephen L
Stem Cell Biology Laboratory, Wolfson Centre for Age-Related Diseases Kings College London, London, UK.
Methods Mol Biol. 2009;481:169-80. doi: 10.1007/978-1-59745-201-4_14.
Use of human hepatocytes for therapeutic and drug discovery applications is hampered by limited tissue source and the inability of hepatocytes to proliferate and maintain function long-term in vitro. Human embryonic stem (hES) cells are immortal and pluripotent and may provide a cell source for functional human hepatocytes (1) Here we have outlined some of the protocols currently in use for the generation of hepatocytes from hES cells.
用于治疗和药物研发的人肝细胞因组织来源有限以及肝细胞在体外无法长期增殖并维持功能而受到阻碍。人胚胎干细胞（hES细胞）具有永生性和多能性，可能为功能性人肝细胞提供细胞来源。（1）在此，我们概述了目前一些用于从hES细胞生成肝细胞的方案。
