Hannover Medical School, Excellence-Cluster REBIRTH, JRG Stem Cell Biology, D-30625, Hannover, Germany.
Biol Chem. 2009 Oct;390(10):1047-55. doi: 10.1515/BC.2009.120.
In regenerative medicine pluripotent stem cells are considered to be a valuable self-renewing source for therapeutic cell transplantations, given that a functional organ-specific phenotype can be acquired by in vitro differentiation protocols. Furthermore, derivatives of pluripotent stem cells that mimic fetal progenitor stages could serve as an important tool to analyze organ development with in vitro approaches. Because of ethical issues regarding the generation of human embryonic stem (ES) cells, other sources for pluripotent stem cells are intensively studied. Like in less developed vertebrates, pluripotent stem cells can be generated from the female germline even in mammals, via parthenogenetic activation of oocytes. Recently, testis-derived pluripotent stem cells were derived from the male germline. Therefore, we compared two different hepatic differentiation approaches and analyzed the generation of definitive endoderm progenitor cells and their further maturation into a hepatic phenotype using murine parthenogenetic ES cells, germline-derived pluripotent stem cells, and ES cells. Applying quantitative RT-PCR, both germline-derived pluripotent cell lines show similar differentiation capabilities as normal murine ES cells and can be considered an alternative source for pluripotent stem cells in regenerative medicine.
在再生医学中，多能干细胞被认为是治疗性细胞移植的有价值的自我更新来源，因为通过体外分化方案可以获得具有器官特异性表型的功能。此外，模拟胎儿祖细胞阶段的多能干细胞衍生物可以作为通过体外方法分析器官发育的重要工具。由于涉及人类胚胎干细胞 (ES) 生成的伦理问题，其他多能干细胞的来源也在被深入研究。与不太发达的脊椎动物一样，多能干细胞甚至可以通过卵母细胞的孤雌生殖激活从雌性生殖系中产生，在哺乳动物中也是如此。最近，睾丸衍生的多能干细胞已从雄性生殖系中产生。因此，我们比较了两种不同的肝分化方法，并使用鼠孤雌生殖 ES 细胞、生殖系衍生的多能干细胞和 ES 细胞分析了确定内胚层祖细胞的生成及其进一步成熟为肝表型。通过定量 RT-PCR，两种生殖系衍生的多能细胞系显示出与正常鼠 ES 细胞相似的分化能力，可被视为再生医学中多能干细胞的替代来源。
