Jeffery Rosemary, Poulsom Richard, Alison Malcolm R
Histopathology Unit, Cancer Research UK, London Research Institute, London, UK.
Methods Mol Biol. 2009;481:141-54. doi: 10.1007/978-1-59745-201-4_12.
Bone marrow cells can engraft in the liver and differentiate into a variety of cell types including hepatocytes and myofibroblasts. This chapter describes how, after transplantation of male bone marrow into female recipients, cells of bone marrow origin (male) can be identified in the female liver by virtue of detection of the Y chromosome by the technique of in situ hybridisation (ISH). Furthermore, ISH for Y chromosome detection can be combined both with immunohistochemistry (IHC) to identify phenotype and with ISH for mRNA to demonstrate function. Additionally, we show that bone marrow-derived cells can be identified in the liver without prior sex-mismatch bone marrow transplantation, identifying instead the BCR:ABL fusion gene that is present in all such cells in almost all patients suffering from chronic myelogenous leukaemia (CML).
骨髓细胞可植入肝脏并分化为多种细胞类型，包括肝细胞和成肌纤维细胞。本章描述了将雄性骨髓移植到雌性受体后，如何通过原位杂交（ISH）技术检测Y染色体，在雌性肝脏中鉴定出骨髓来源的（雄性）细胞。此外，用于检测Y染色体的ISH可与免疫组织化学（IHC）相结合以鉴定表型，还可与用于mRNA的ISH相结合以证明功能。此外，我们表明，无需事先进行性别不匹配的骨髓移植，就可以在肝脏中鉴定出骨髓来源的细胞，而是鉴定几乎所有慢性粒细胞白血病（CML）患者所有此类细胞中都存在的BCR:ABL融合基因。
