Su Yingjuan, Wang Ting, Sun Yufei, Ye Huagu
State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, P.R. China.
Biochem Genet. 2009 Feb;47(1-2):56-65. doi: 10.1007/s10528-008-9206-2. Epub 2008 Dec 19.
Euryodendron excelsum is a critically endangered Ternstroemiaceae species endemic to southern China, with only 14 individuals surviving in Ba Jia Zhen of Yangchun, Guangdong Province. Intersimple sequence repeat (ISSR) markers were used to assess genetic variation and relationships among these individuals. Population genetic parameters were estimated by a Bayesian approach as well as conventional methods. Of the 225 loci generated by 21 primers, 147 (65.33%) were polymorphic. Compared with other species of Theaceae and related families, a high level of genetic variation was identified in E. excelsum (Nei's gene diversity, 0.2458; Shannon's index, 0.3626). An unweighted pair group method with arithmetic mean (UPGMA) dendrogram showed that the 14 individuals were mainly clustered into three groups, a conclusion further supported by principal coordinate analysis. Based on these results, a management and conservation strategy for E. excelsum was proposed.
猪血木是中国南方特有的山茶科极危物种，仅存于广东省阳春市八甲镇的14株个体。利用简单序列重复区间（ISSR）标记评估这些个体间的遗传变异和关系。采用贝叶斯方法及传统方法估计群体遗传参数。21条引物产生的225个位点中，147个（65.33%）具有多态性。与山茶科其他物种及相关科相比，猪血木具有较高水平的遗传变异（Nei氏基因多样性，0.2458；香农指数，0.3626）。算术平均数的非加权成组配对法（UPGMA）聚类图显示，14个个体主要聚为三组，主坐标分析进一步支持了这一结论。基于这些结果，提出了猪血木的管理和保护策略。
