Sylla Niang M, Bombled C, Schlumberger M, Bidart J M
Faculté de Médecine, Pharmacie et Odontologie, Département de Sciences biologiques et pharmaceutiques appliquées, Service d'Immunologie, Université Cheikh Anta DIOP de Dakar, Sénégal.
Dakar Med. 2007;52(3):225-30.
Serum thyroglobulin measurements play an integral role in clinical evaluation of patients with thyroid cancer. Serum thyroglobulin is a highly specific and sensitive tumor marker for detecting persistent or recurrent thyroid cancer but also for monitoring clinical status. Actually, chemiluminescent methods gain ground on the radioimmunological methods because they offer the practical advantage of a shorter incubation time, a wider range of measured values and a reagent marked antibody more stable, less fragile than those used on radioimmunoassay. The aim of this study was to compare, by correlation study, three chemiluminescent methods to the reference radioimmunological method usually used in laboratories.
Thyroglobulin was measured in 203 patients by the 3 following analyzers: Nichols Advantage (Nichols Institute Diagnostics, CA, USA), Immulite 2000 ( DPC Roche, Siemens, Los Angeles, USA) and Elecsys 2010 (Roche Diagnostics, Manheim, Germany); and by manual method (SELco Tg (Medipan Diagnostica, Berlin, Germany). Correlation analysis with OriginLab software version 7.0 was performed in order to compare thyroglobulin distribution values measured by the different methods.
Correlation coefficients obtained were for Medipan/ Immulite 2000: 0.95 (n = 80); for Medipan/Elecsys: 0.97 (n = 31); for Medipan/Advantage: r = 0.96 (n = 73).
Chemioluminescent technics we studied could be validly used in patients without anti-thyroglobulin antibody. The correlation coefficients we obtained allow us to select one of these automated methods after their performance was studied.
血清甲状腺球蛋白检测在甲状腺癌患者的临床评估中起着不可或缺的作用。血清甲状腺球蛋白是检测持续性或复发性甲状腺癌以及监测临床状态的一种高度特异性和敏感性的肿瘤标志物。实际上，化学发光法正逐渐取代放射免疫法，因为它具有孵育时间短、测量值范围广以及标记抗体试剂更稳定、比放射免疫分析中使用的试剂更不易损坏等实际优势。本研究的目的是通过相关性研究，将三种化学发光法与实验室通常使用的参考放射免疫法进行比较。
采用以下三种分析仪对203例患者的甲状腺球蛋白进行检测：Nichols Advantage（美国加利福尼亚州Nichols研究所诊断公司）、Immulite 2000（美国洛杉矶DPC罗氏公司，西门子）和Elecsys 2010（德国曼海姆罗氏诊断公司）；并采用手工方法（SELco Tg，德国柏林Medipan诊断公司）。使用OriginLab软件7.0版进行相关性分析，以比较不同方法测量的甲状腺球蛋白分布值。
Medipan/Immulite 2000的相关系数为0.95（n = 80）；Medipan/Elecsys的相关系数为0.97（n = 31）；Medipan/Advantage的相关系数为r = 0.96（n = 73）。
我们研究的化学发光技术可有效地用于无抗甲状腺球蛋白抗体的患者。我们获得的相关系数使我们能够在研究其性能后选择其中一种自动化方法。
