Mao Hui-Juan, Wu Hui-Hui, Bu Lin-Ling, Zhou Yu-Bao, Sun Jia, Sun Ping-Long
College of Acu-moxibustion and Massage, Shanghai University of Chinese Medicine, Shanghai 201203, China.
Zhen Ci Yan Jiu. 2008 Oct;33(5):291-5.
To study the underlining mechanism of electroacupuncture (EA) in regulating leukocyte counts in the rabbit.
A total of 75 rabbits were randomly divided into normal control, leukocytosis model, leukocytosis + EA, leukopenia model, leukopenia+EA groups, with 15 cases in each. EA (2-20 Hz, 1-2 mA) was applied to "Dazhui" (DU 14), bilateral "Zusanli" (ST 36) and bilateral "Geshu" (BL 17) for 20 min, once daily for 5 days. Leukocytosis and leukopenia models were duplicated by intravenous injection of coliform (0.1 ml/kg) and cyclophosphamide (30 mg/kg) separately. Peripheral blood leukocyte counts were detected every day. At the end of the experiment, the rabbit's spleen tissue was collected under anesthesia, and the diameter of splenic sinusoid basal lamina eyehole was measured under scanning electronic microscope.
Compared with normal control group, after i.v. coliform and cyclophosphamide, leukocytes increased and decreased significantly and respectively, while the area of the splenic sinusoid basal lamina eyehole in leukocytosis model group increased lightly and that of leukopenia model group reduced obviously (P < 0.01). In comparison with their individual model group, the leukocyte count in leukocytosis + EA group decreased considerably (P < 0.05), while that of leukopenia + EA group increased markedly (P < 0.01). The area of splenic sinusoid basal lamina eyehole in leukocytosis+ EA group decreased apparently (P < 0.05), and that in leukopenia+ EA group increased remarkably (P < 0.05). CONCLUSION EA is able to regulate the leukocyte counts in coliform-induced leukocytosis and cyclophosphamide-induced leukopenia rabbits, which may be related with its effects in improving the splenic sinusoid basal lamina eyehole activity.
研究电针调节家兔白细胞计数的潜在机制。
将75只家兔随机分为正常对照组、白细胞增多症模型组、白细胞增多症+电针组、白细胞减少症模型组、白细胞减少症+电针组，每组15只。采用2 - 20Hz、1 - 2mA的电针刺激“大椎”（督脉14）、双侧“足三里”（足阳明胃经36）和双侧“膈俞”（足太阳膀胱经17）20分钟，每日1次，共5天。分别通过静脉注射大肠杆菌（0.1ml/kg）和环磷酰胺（30mg/kg）复制白细胞增多症和白细胞减少症模型。每天检测外周血白细胞计数。实验结束时，麻醉下采集家兔脾脏组织，在扫描电子显微镜下测量脾血窦基膜小孔直径。
与正常对照组相比，静脉注射大肠杆菌和环磷酰胺后，白细胞分别显著增多和减少，白细胞增多症模型组脾血窦基膜小孔面积轻度增加，白细胞减少症模型组明显减小（P < 0. 01）。与各自模型组相比，白细胞增多症+电针组白细胞计数显著降低（P < 0. 05），白细胞减少症+电针组显著升高（P < 0. 01）。白细胞增多症+电针组脾血窦基膜小孔面积明显减小（P < 0. 05），白细胞减少症+电针组明显增加（P < 0. 05）。结论电针能够调节大肠杆菌诱导的白细胞增多症和环磷酰胺诱导的白细胞减少症家兔的白细胞计数，这可能与其改善脾血窦基膜小孔活性的作用有关。
