Shen Rong, Xu Ying, Zhang Zhi-Xiong, Li Yun, Wang Xing-Yu
The Affiliated Renji Hospital of Shanghai Jiaotong University, Shanghai 200001, China.
Zhen Ci Yan Jiu. 2009 Oct;34(5):329-33.
To observe the effect of electroacupuncture (EA) combined with Ginkgo biloba extract (GBE 50) on learning-memory ability and hippocampal cytokine contents in aging rats for exploring its underlying mechanism in the treatment of dysmnesy.
Forty-five SD rats were randomly divided into control (n=9), model (n=8), EA (n=10), GBE 50 (n=9) and EA + GBE 50 (n=9) groups. The dysmnesy model was established by D-galactose intraperitoneal injection for 42 days. EA (3 Hz, 1 mA) was applied to "Baihui" (GV 20) and "Zusanli" (ST 36) for 20 min, once every other day for 21 days. The learning-memory ability was detected by Morris water maze tests. The levels of IL-1beta, IL-6 and TNF-alpha in hippocampus were examined by radioimmunoassay.
Compared with control group, the mean escape latency (MEL) of the rats in model group was significantly greater on the 2nd and 3rd day training (P<0.05, P<0.01), and the percent of swimming distance (PSD) in the target quadrant was shortened significantly (P<0.01). Compared with model group, the MEL values of the rats in EA, GBE 50 and EA + GBE 50 groups were significantly shortened (P<0.05, P<0.01), and the PSD values of the later 3 groups increased considerably (P<0.01). Comparison among the EA, GBE 50 and EA + GBE 50 groups showed that the MEL of EA + GBE 50 was obviously shorter than those of EA and GBE 50 groups (P<0.05). Compared with control group, the contents of IL-1beta) and TNF-alpha in hippocampus in model group increased significantly, but IL-6 decreased markedly (P<0.05, P<0.01). In comparison with model group, the IL-1beta contents of EA, GBE 50 and EA + GBE 50 groups, and TNF-alpha of EA and EA + GBE 50 groups were reduced significantly (P<0.05, P<0.01); and the contents of IL-6 in GBE 50 and EA + GBE 50 groups increased apparently (P<0.01). No significant differences were found between model and EA groups in IL-6 levels, and between model and EA + GBE 50 groups in hippocampal TNF-a levels (P>0.05).
Both EA and GBE 50 can improve the dysmnesy rats' learning-memory ability, which may be closely associated with their effects in regulating hippocampal IL-1beta, IL-6 and TNF-alpha levels to relieve the inflammatory reaction. Combined administration of EA and GBE 50 has a synergic effect.
观察电针（EA）联合银杏叶提取物（GBE 50）对衰老大鼠学习记忆能力及海马细胞因子含量的影响，探讨其治疗记忆障碍的潜在机制。
将45只SD大鼠随机分为对照组（n = 9）、模型组（n = 8）、电针组（n = 10）、GBE 50组（n = 9）和电针 + GBE 50组（n = 9）。通过腹腔注射D - 半乳糖42天建立记忆障碍模型。将电针（3Hz，1mA）应用于“百会”（GV 20）和“足三里”（ST 36）20分钟，隔日1次，共21天。通过Morris水迷宫试验检测学习记忆能力。采用放射免疫法检测海马中IL - 1β、IL - 6和TNF - α的水平。
与对照组相比，模型组大鼠在训练第2天和第3天的平均逃避潜伏期（MEL）显著延长（P < 0.05，P < 0.01），目标象限游泳距离百分比（PSD）显著缩短（P < 0.01）。与模型组相比，电针组、GBE 50组和电针 + GBE 50组大鼠的MEL值显著缩短（P < 0.05，P < 0.01），后3组的PSD值显著增加（P < 0.01）。电针组、GBE 50组和电针 + GBE 50组之间比较，电针 + GBE 50组的MEL明显短于电针组和GBE 50组（P < 0.05）。与对照组相比，模型组海马中IL - 1β和TNF - α含量显著增加，但IL - 6明显降低（P < 0.05，P < 0.01）。与模型组相比，电针组、GBE 50组和电针 + GBE 50组的IL - 1β含量，以及电针组和电针 + GBE 50组的TNF - α含量显著降低（P < 0.05，P < 0.01）；GBE 50组和电针 + GBE 50组的IL - 6含量明显增加（P < 0.01）。模型组与电针组的IL - 6水平之间，以及模型组与电针 + GBE 50组的海马TNF - α水平之间无显著差异（P > 0.05）。
电针和GBE 50均可改善记忆障碍大鼠的学习记忆能力，这可能与其调节海马IL - 1β、IL - 6和TNF - α水平以减轻炎症反应的作用密切相关。电针与GBE 50联合应用具有协同作用。
