Ji Lai-Xi, Yan Li-Ping, Wang Hai-Jun, Wang Bo, Zhang Xia-Yi, Zhang Tian-Sheng, Jin Xiao-Fei
Department of Acu-moxibustion, Shanxi University of Traditional Chinese Medicine, Taiyuan 030024, China.
Zhen Ci Yan Jiu. 2008 Oct;33(5):296-300, 325.
To select the best combination of the commonly-used acupoints [Zhongwan (CV 12), Neiguan (PC 6), and Zusanli (ST 36)] for treatment of gastric disorders.
Forty-eight Wistar rats were randomly divided into 8 groups: model, ST36, CV12, PC6, ST36 + PC6, ST36 + CV12, CV12 + PC6 and ST36 + CV12 + PC6 by factorial design method. Acute gastric mucosal lesion model was established by intragastric perfusion of anhydrous alcohol. EA(1- 3 mA, 30 Hz) was applied to the above-mentioned acupoints for 20 min respectively. And after EA, gastric mucosal lesion index (LI), pathological lesion integral score (PLIS) were calculated and mucosal ultramicrostructural changes observed by optical microscope and transmission electron microscope respectively.
Compared with model group, the gastric mucosal LI and PLIS of ST36, CV12, PC6, ST36 + CV12, ST36 + PC6, CV12 + PC6, ST36 + CV12 + PC6 groups all decreased significantly (P < 0.05). Comparison among 7 EA groups showed that both LI and PLIS of ST36 + CV12 + PC6 group were significantly lower than those of ST36, V12, PC6, ST36 + CV12, ST36 + PC6 and CV12 + PC6 groups (P < 0.05). No significant differences were found between every two groups of ST36, CV12, PC6, ST36+ CV12, ST36 + PC6 and CV12 + PC6 groups (P > 0.05) in both LI and PLIS. Ultrastructural results showed that the injury of gastric mucosal parietal cells and delomorphous cells including mitochondrial swelling, crista arrange disorder and breaking, enlargement of the rough endoplasmic reticulum, ribosome exfoliation, etc. in model group was serious, but lightened significantly in EA groups particularly in ST36 + CV12 + PC6 group.
ST36, PC6 and CV12 combination can effectively reduce anhydrous alcohol-induced gastric mucosal lesion in rats, and may be used as a basic formula
筛选治疗胃部疾病常用穴位[中脘（CV12）、内关（PC6）和足三里（ST36）]的最佳组合。
采用析因设计方法将48只Wistar大鼠随机分为8组：模型组、ST36组、CV12组、PC6组、ST36+PC6组、ST36+CV12组、CV12+PC6组和ST36+CV12+PC6组。通过灌胃无水乙醇建立急性胃黏膜损伤模型。分别对上述穴位施加电针（1 - 3 mA，30 Hz）20分钟。电针后，分别计算胃黏膜损伤指数（LI）、病理损伤积分（PLIS），并通过光学显微镜和透射电子显微镜观察黏膜超微结构变化。
与模型组相比，ST36组、CV12组、PC6组、ST36+CV12组、ST36+PC6组、CV12+PC6组、ST36+CV12+PC6组的胃黏膜LI和PLIS均显著降低（P<0.05）。7个电针组比较显示，ST36+CV12+PC6组的LI和PLIS均显著低于ST36组、CV12组、PC6组、ST36+CV12组、ST36+PC6组和CV12+PC6组（P<0.05）。ST36组、CV12组、PC6组、ST36+CV12组、ST36+PC6组和CV12+PC6组两两之间的LI和PLIS均无显著差异（P>0.05）。超微结构结果显示，模型组胃黏膜壁细胞和主细胞损伤严重，包括线粒体肿胀、嵴排列紊乱和断裂、粗面内质网扩张、核糖体脱落等，但电针组尤其是ST36+CV12+PC6组损伤明显减轻。
ST36、PC6和CV12联合应用能有效减轻无水乙醇诱导的大鼠胃黏膜损伤，可作为基础配方
