Olander R M, Muotiala A, Himanen J P, Karvonen M, Airaksinen U, Runeberg-Nyman K
Molecular Biology Unit, National Public Health Institute, Helsinki, Finland.
Microb Pathog. 1991 Feb;10(2):159-64. doi: 10.1016/0882-4010(91)90076-m.
Pertussis toxin (PT) subunit S1 was produced in Bacillus subtilis as a secretory protein designated BacS1. BacS1 was partially purified and used to immunize mice. The sera were tested for PT-neutralizing antibodies and for protective capacity in a mouse model. Unlike previous findings with recombinant S1 from Escherichia coli, the recombinant BacS1 protein induced antibodies that were both neutralizing and protective. An adjuvant was necessary for efficient immunization with BacS1 but not with PT. Of the four adjuvants tested, aluminium phosphate gel was insufficient whereas Freund's incomplete adjuvant, Klebsiella lipopolysaccharide and Ribi's monophosphoryl lipid A-trehalose dimycolate emulsion all resulted in protective antibody production in NIH mice.
百日咳毒素（PT）亚基S1在枯草芽孢杆菌中作为一种名为BacS1的分泌蛋白产生。BacS1被部分纯化并用于免疫小鼠。检测血清中的PT中和抗体以及在小鼠模型中的保护能力。与之前从大肠杆菌获得的重组S1的研究结果不同，重组BacS1蛋白诱导产生了具有中和作用和保护作用的抗体。用BacS1进行有效免疫需要佐剂，但用PT则不需要。在所测试的四种佐剂中，磷酸铝凝胶效果不佳，而弗氏不完全佐剂、肺炎克雷伯菌脂多糖和Ribi的单磷酰脂质A - 海藻糖二霉菌酸酯乳液均能在NIH小鼠中诱导产生保护性抗体。
