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通过电刺激采精法对圈养南美浣熊(Nasua nasua)精液特征及精子细胞超微结构的测定

Determination of semen characteristics and sperm cell ultrastructure of captive coatis (Nasua nasua) collected by electroejaculation.

作者信息

Lima Gabriela L, Barros Felipe F P C, Costa Leonardo L M, Castelo Thibério S, Fontenele-Neto José D, Silva Alexandre R

机构信息

Department of Animal Science, Country Federal University of Semi-Arid - UFERSA, BR 110, Km 47, Presidente Costa e Silva, 59625-900, Mossoró, RN, Brazil.

出版信息

Anim Reprod Sci. 2009 Oct;115(1-4):225-30. doi: 10.1016/j.anireprosci.2008.11.003. Epub 2008 Nov 18.

DOI:10.1016/j.anireprosci.2008.11.003
PMID:19097713
Abstract

Despite the wide geographical distribution of coati (Nasua nasua) from the south of Canada to the north of Argentina, studies regarding the reproductive characteristics of this species are extremely limited. The objective of this study was to describe the various characteristics of coati semen by morphometric and ultrastructural analysis. Five mature males were anesthetized and electroejaculated for the collection of semen. Semen was immediately evaluated for color, volume, pH, sperm motility, vigor, morphology, acrosomal integrity, percentage of live cells and hypo-osmotic response by light microscopy. Sperm cell morphometry and ultrastructural analyses were also performed. Observations of seminal characteristics determined by electroejaculation in captive coatis represent a valuable baseline dataset for establishing fertility standards and provide background information that may be useful for assisted breeding programmes in members of the Procyonidae family.

摘要

尽管浣熊(南美浣熊)的地理分布广泛,从加拿大南部到阿根廷北部都有,但关于该物种繁殖特征的研究极为有限。本研究的目的是通过形态计量学和超微结构分析来描述浣熊精液的各种特征。对五只成年雄性浣熊进行麻醉并通过电刺激射精来采集精液。精液立即通过光学显微镜对颜色、体积、pH值、精子活力、活力、形态、顶体完整性、活细胞百分比和低渗反应进行评估。还进行了精子细胞形态计量学和超微结构分析。通过电刺激射精对圈养浣熊精液特征的观察,为建立生育标准提供了有价值的基础数据集,并提供了可能对浣熊科动物辅助繁殖计划有用的背景信息。

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