Laboratory of Carnivore Reproduction, UECE, Paranjana Ave, 1700, Itaperi, 60740-000, Fortaleza, CE, Brazil.
Anim Reprod Sci. 2010 Apr;118(2-4):362-5. doi: 10.1016/j.anireprosci.2009.08.012. Epub 2009 Aug 25.
The aim of this study is to describe the characteristics of the semen from six-banded armadillos (Euphractus sexcinctus) collected by electroejaculation. Six mature males were physically restrained and electroejaculated twice for the collection of semen. Semen collected was immediately evaluated for appearance, volume, pH, sperm motility, vigor, morphology, percentage of live sperm and functional membrane integrity by light microscopy. Semen was obtained from all (100%) twelve attempts conducted for electroejaculation. Armadillos' semen had a white-translucent appearance, and great viscosity. Mean values obtained in analysis of the semen were: 353+/-86 microl for volume, 9 for pH, 45+/-14 x 10(6)sperm/ml for concentration, 61+/-7% motile sperm with 2+/-0.2 for vigor, 55+/-7% live sperm, 86+/-2% morphologic normal sperm, and 46+/-6% functional membrane integrity. In conclusion, semen from six-banded armadillos can be efficiently obtained by electroejaculation. The characteristics of semen collected by electroejaculation in six-banded armadillos provide background information that may be useful for assisted breeding programs in the members of the Xenarthra family.
本研究旨在描述电刺激采集的六带犰狳(Euphractus sexcinctus)精液的特征。对 6 只成熟雄性六带犰狳进行了物理约束,并进行了两次电刺激以收集精液。收集的精液立即通过光学显微镜评估外观、体积、pH 值、精子活力、活力、形态、活精子百分比和功能膜完整性。电刺激采集共进行了 12 次尝试,均获得了精液。犰狳精液呈白色半透明状,且具有很强的粘性。精液分析的平均值为:体积 353+/-86 微升,pH 值 9,浓度 45+/-14 x 10(6)sperm/ml,活力 2+/-0.2 的可运动精子 61+/-7%,活精子 55+/-7%,形态正常精子 86+/-2%,功能膜完整性 46+/-6%。总之,电刺激可以有效地从六带犰狳中获得精液。电刺激采集的六带犰狳精液的特征为奇蹄目动物的辅助繁殖计划提供了有用的背景信息。