Birkmann Eva, Riesner Detlev
Institut für Physikalische Biologie, Heinrich-Heine-Universitaet Duesseldorf & Institut für Biophysik und Neurowissenschaften, Forschungszentrum Juelich, Duesseldorf, Germany.
Prion. 2008 Apr-Jun;2(2):67-72. doi: 10.4161/pri.2.2.7060. Epub 2008 Apr 23.
The prion infection is a conversion of host encoded prion protein (PrP) from its cellular isoform PrP(C) into the pathological and infectious isoform PrP(Sc); the conversion process was investigated by in vitro studies using recombinant and cellular PrP and natural PrP(Sc). We present a brief summary of the results determined with our in vitro conversion system and the derived mechanistic models. We describe well characterized intermediates and precursor states during the conversion process, kinetic studies of spontaneous and seeded fibrillogenesis and the impact of the membrane environment.
朊病毒感染是将宿主编码的朊病毒蛋白（PrP）从其细胞异构体PrP(C)转化为病理性和传染性异构体PrP(Sc)；利用重组PrP、细胞PrP和天然PrP(Sc)通过体外研究对该转化过程进行了探究。我们简要总结了用我们的体外转化系统所确定的结果以及推导的机制模型。我们描述了转化过程中特征明确的中间体和前体状态、自发和接种的纤维形成的动力学研究以及膜环境的影响。
