Yamamori T
Biology Division, California Institute of Technology, Pasadena 91125.
Neuroreport. 1991 Apr;2(4):173-6. doi: 10.1097/00001756-199104000-00003.
We recently demonstrated that the neuronal cholinergic differentiation factor (CDF) which switches the neurotransmitter phenotype of cultured sympathetic neurons from noradrenergic to cholinergic is identical to leukemia inhibitory factor (LIF). To elucidate some of the initial events leading to the phenotypic switch, the effects of CDF/LIF on the mRNA levels of several immediate early genes were examined in cultured neonatal rat sympathetic neurons. c-fos and jun-B were induced within 30 min of addition of CDF/LIF. In contrast, no effect on the expression of c-myc, fra-1, v-jun or actin mRNA was detected at this time. Thus, CDF/LIF may induce the expression of particular immediate early genes prior to its positive and negative effects on neurotransmitter and neuropeptide gene expression.
我们最近证明，能将培养的交感神经元的神经递质表型从去甲肾上腺素能转换为胆碱能的神经元胆碱能分化因子（CDF）与白血病抑制因子（LIF）是相同的。为了阐明导致表型转换的一些初始事件，我们在培养的新生大鼠交感神经元中检测了CDF/LIF对几个立即早期基因mRNA水平的影响。添加CDF/LIF后30分钟内，c-fos和jun-B被诱导表达。相比之下，此时未检测到对c-myc、fra-1、v-jun或肌动蛋白mRNA表达有影响。因此，CDF/LIF可能在其对神经递质和神经肽基因表达产生正负效应之前，先诱导特定立即早期基因的表达。
