生物活性Wnt蛋白的分离与应用

Isolation and application of bioactive Wnt proteins.

作者信息

Willert Karl H

机构信息

Cellular and Molecular Medicine, University of California-San Diego, La Jolla, USA.

出版信息

Methods Mol Biol. 2008;468:17-29. doi: 10.1007/978-1-59745-249-6_2.

DOI:10.1007/978-1-59745-249-6_2

PMID:19099243

Abstract

Wnt proteins and their signaling cascades are involved in a wide variety of developmental processes, and deregulation of this pathway is frequently associated with tumorigenesis. Unlike many other growth factors, Wnts long eluded biochemical purification, in large part because of their hydrophobic nature, which is imparted by one or more lipid modifications. Here I describe a complete protocol that outlines the purification process for Wnt proteins. While this protocol has not been applied to all known Wnt proteins, it has been successfully applied to the purification of a large subset of Wnts, including the very divergent Wnt protein, Drosophila Wnt8 (Dwnt8 or WntD), indicating that this protocol is likely applicable to all Wnts.

摘要

Wnt蛋白及其信号级联反应参与多种发育过程，该信号通路失调常与肿瘤发生相关。与许多其他生长因子不同，Wnt蛋白长期以来难以进行生化纯化，这在很大程度上是由于其疏水性质，这种性质是由一种或多种脂质修饰赋予的。在此，我描述了一个完整的方案，概述了Wnt蛋白的纯化过程。虽然该方案尚未应用于所有已知的Wnt蛋白，但已成功应用于纯化大部分Wnt蛋白，包括差异很大的Wnt蛋白——果蝇Wnt8（Dwnt8或WntD），这表明该方案可能适用于所有Wnt蛋白。