Bogdanova Vera S, Galieva Elvira R, Kosterin Oleg E
Institute of Cytology and Genetics, Siberian Department of Russian Academy of Sciences, acad. Lavrentiev av., 10, 630090 Novosibirsk, Russia.
Theor Appl Genet. 2009 Feb;118(4):801-9. doi: 10.1007/s00122-008-0940-y. Epub 2008 Dec 20.
The genetic basis of nuclear-cytoplasmic incompatibility was examined using the wild pea (Pisum sativum subsp. elatius) accession VIR320. When this accession is used as the female parent in crosses with domesticated peas (Pisum sativum subsp. sativum) the F(1) is highly sterile and displays chlorophyll deficiency, chlorophyll variegation, reduction of leaflets and stipulae while the reciprocal cross produces hybrids that appear normal. A mapping recombinant inbred line (RIL) population was established based on a cross in a compatible direction of a tester line WL1238 with VIR320. The ability to cause nuclear-cytoplasmic conflict was analysed by crossing individual RIL plants as pollen parents with VIR320 as donor of cytoplasm and scoring each F(1) for major signs of the conflict. It is concluded that two unlinked nuclear genes are involved in the genetic control of the observed incompatibility. One of the genes, denoted as Scs1, is closely linked to the PhlC gene on linkage group III and the other, denoted as Scs2, is closely linked to the gp gene on linkage group V. Alleles of both genes in WL1238 are dominant and appear to be lethal in the homozygous condition in the VIR320 cytoplasm background.
利用野生豌豆（Pisum sativum subsp. elatius）种质VIR320研究了核质不亲和性的遗传基础。当该种质在与栽培豌豆（Pisum sativum subsp. sativum）杂交中用作母本时，F(1)高度不育，并表现出叶绿素缺乏、叶绿素斑驳、小叶和托叶减少，而反交产生的杂种看起来正常。基于测试系WL1238与VIR320在相容方向上的杂交建立了一个定位重组自交系（RIL）群体。通过将单个RIL植株作为花粉亲本与作为细胞质供体的VIR320杂交，并对每个F(1)的冲突主要特征进行评分，分析了引起核质冲突的能力。得出的结论是，两个不连锁的核基因参与了所观察到的不亲和性的遗传控制。其中一个基因，记为Scs1，与连锁群III上的PhlC基因紧密连锁，另一个基因，记为Scs2，与连锁群V上的gp基因紧密连锁。WL1238中这两个基因的等位基因都是显性的，并且在VIR320细胞质背景下纯合状态时似乎是致死的。
