Department of Botany, Faculty of Sciences, Palacký University, 78371 Olomouc, Czech Republic.
Department of Geoinformatics, Faculty of Sciences, Palacký University, 78371 Olomouc, Czech Republic.
Int J Mol Sci. 2019 Apr 10;20(7):1773. doi: 10.3390/ijms20071773.
Reproductive isolation is an important component of species differentiation. The plastid gene coding for the acetyl-CoA carboxylase subunit and the nuclear gene coding for the biotin carboxyl carrier protein were identified as candidate genes governing nuclear-cytoplasmic incompatibility in peas. We examined the allelic diversity in a set of 195 geographically diverse samples of both cultivated ( , ) and wild ( and ) peas. Based on deduced protein sequences, we identified 34 and 31 alleles that are partially geographically and genetically structured. The is highly variable due to insertions of tandem repeats. and have unique alleles and combinations of both genes. On the other hand, partial overlap was observed between and . Mapping of protein sequence polymorphisms to 3D structures revealed that most of the repeat and indel polymorphisms map to sequence regions that could not be modeled, consistent with this part of the protein being less constrained by requirements for precise folding than the enzymatically active domains. The results of this study are important not only from an evolutionary point of view but are also relevant for pea breeding when using more distant wild relatives.
生殖隔离是物种分化的一个重要组成部分。质体基因编码乙酰辅酶 A 羧化酶亚基和核基因编码生物素羧基载体蛋白被鉴定为豌豆核质不亲和的候选基因。我们在一组 195 个具有地理多样性的栽培（ 和野生（ 和 ）豌豆样本中检查了等位基因多样性。基于推导的蛋白质序列，我们鉴定了 34 个 和 31 个部分具有地理和遗传结构的等位基因。由于串联重复的插入，高度可变。 和 具有独特的等位基因和两者的组合。另一方面， 和 之间存在部分重叠。蛋白质序列多态性与 3D 结构的映射表明，大多数重复和缺失多态性映射到无法建模的序列区域，这与该蛋白质的这部分比酶活性结构域更不受精确折叠要求的限制相一致。这项研究的结果不仅从进化的角度来看很重要，而且在利用更远缘的野生亲属进行豌豆育种时也很重要。
