Yang R, Xia T, Xu G, Li Z, Ying Z, Xu X
Department of Clinical Laboratory, Peking University First Hospital, Beijing, China.
Transplant Proc. 2008 Dec;40(10):3500-4. doi: 10.1016/j.transproceed.2008.03.176.
Our study sought to investigate the immune response of cytotoxic T lymphocytes to human cytomegalovirus (HCMV) in kidney transplant recipients with virus reactivation. Nine kidney transplant recipients with serum HCMV immunoglobulin G and HLA-A 0201 genotype displayed HCMV pp65 antigenemia and were compared with 29 control recipients who were free of antigenemia. The frequency of total HCMV-specific cytotoxic T cells was determined using HLA-A2-NLVPMVATV-pentamer staining. The frequency of HCMV specific interferon (INF)-gamma secreting CD8(+) T cells was determined using intracellular INF-gamma staining after NLVPMVATV pulsing in vitro. The proportion of IFN-gamma secreting pentamer-stained CD8(+) T cells was calculated, representing the capacity for INF-gamma secretion among HCMV-specific CD8(+) T cells. We observed that the frequency of HCMV-specific pentamer-stained CD8(+) T cells and HCMV-specific IFN-gamma secreting CD8(+) T cells both remained unchanged, yet the proportion of IFN-gamma secreting CD8(+) T cells showed a significant difference between the 2 groups (39.2% +/- 17.2% vs 20.0% +/- 13.3% respectively; P = .004), reflecting impaired IFN-gamma secreting capacity of HCMV-specific CD8(+) T cells among kidney transplant recipients displaying antigenemia, which may be a crucial reason for HCMV reactivation in immunosuppressed patients.
我们的研究旨在调查肾移植受者病毒再激活时细胞毒性T淋巴细胞对人巨细胞病毒（HCMV）的免疫反应。9名血清HCMV免疫球蛋白G和HLA - A 0201基因型的肾移植受者出现HCMV pp65抗原血症，并与29名无抗原血症的对照受者进行比较。使用HLA - A2 - NLVPMVATV - 五聚体染色法测定总HCMV特异性细胞毒性T细胞的频率。在体外经NLVPMVATV刺激后，使用细胞内γ干扰素（INF - γ）染色法测定HCMV特异性分泌INF - γ的CD8(+) T细胞的频率。计算分泌INF - γ的五聚体染色CD8(+) T细胞的比例，代表HCMV特异性CD8(+) T细胞中INF - γ的分泌能力。我们观察到，HCMV特异性五聚体染色CD8(+) T细胞和HCMV特异性分泌INF - γ的CD8(+) T细胞的频率均保持不变，但两组之间分泌INF - γ的CD8(+) T细胞比例存在显著差异（分别为39.2%±17.2%和20.0%±13.3%；P = .004），这反映出在出现抗原血症的肾移植受者中，HCMV特异性CD8(+) T细胞分泌INF - γ的能力受损，这可能是免疫抑制患者中HCMV再激活的关键原因。
